Schneider B, Ambrosius H
Laboratory of Immunobiology, Karl Marx University, Leipzig, GDR.
Biomed Biochim Acta. 1989;48(1):159-63.
Surface immunoglobulin-bearing cells from peripheral blood, thymus, head kidney, and spleen in carp (Cyprinus carpio L.) were studied by immunoperoxidase labelling using antisera against fragments of carp IgM. Different lymphoid cell populations could be determined. Thus, about 35% of the lymphocytes of the peripheral blood, head kidney, and spleen were determined, as "B" lymphocytes, using rabbit anti-carp Fab antiserum with L chain specificity. Nearly all lymphocytes of the peripheral blood, head kidney, and spleen are bearing immunoglobulin determinants or cross-reactive structures. The latter also holds true for about 98% of thymocytes. The differences between the Ig-like surface structures of the thymocytes and the membrane-bound immunoglobulins of a population of lymphocytes are discussed.
利用抗鲤鱼IgM片段的抗血清，通过免疫过氧化物酶标记法研究了鲤鱼（Cyprinus carpio L.）外周血、胸腺、头肾和脾脏中携带表面免疫球蛋白的细胞。可以确定不同的淋巴细胞群体。因此，使用具有L链特异性的兔抗鲤鱼Fab抗血清，外周血、头肾和脾脏中约35%的淋巴细胞被确定为“B”淋巴细胞。外周血、头肾和脾脏中几乎所有淋巴细胞都携带免疫球蛋白决定簇或交叉反应结构。胸腺细胞中约98%也是如此。讨论了胸腺细胞的Ig样表面结构与一群淋巴细胞的膜结合免疫球蛋白之间的差异。
