Li Tai-Yuan, Yin Ping, Zhou Yu, Zhang Yi, Zhang Yong-Ying, Chen Tseh-an
Department of Biotechnology, College of Life Sciences, Wuhan University, Hubei 430072, PR China.
FEMS Microbiol Lett. 2004 Jul 1;236(1):33-9. doi: 10.1016/j.femsle.2004.05.014.
The 4992-bp replicon of a large cryptic plasmid in the gram-positive bacterium Leifsonia xyli subsp. cynodontis was identified and sequenced. The replicon encoded two proteins essential for plasmid replication and stability. The putative replication protein (RepA) is homologous to that of the plasmids in mycobacterial pLR7 family, while the putative ParA protein immediately downstream of RepA is significantly homologous to the Walker-type ATPase required for partition of plasmid and chromosome of the gram-positive bacteria. These two proteins and other ORFs are clustered with the putative promoters and other regulatory sequences, illustrating an efficient organization of the replicon for this novel plasmid.
在革兰氏阳性细菌嗜犬利夫松氏菌（Leifsonia xyli subsp. cynodontis）中，一个大型隐蔽质粒的4992碱基对复制子被鉴定并测序。该复制子编码了两个对质粒复制和稳定性至关重要的蛋白质。假定的复制蛋白（RepA）与分枝杆菌pLR7家族质粒的复制蛋白同源，而紧接在RepA下游的假定ParA蛋白与革兰氏阳性细菌质粒和染色体分配所需的沃克型ATP酶具有显著同源性。这两种蛋白质和其他开放阅读框与假定的启动子和其他调控序列聚集在一起，说明了该新型质粒复制子的高效组织。
