瘤胃乳酸利用细菌中不同的限制与修饰表型。

Different restriction and modification phenotypes in ruminal lactate-utilizing bacteria.

作者信息

Piknova Maria, Filova Miroslava, Javorsky Peter, Pristas Peter

机构信息

Faculty of Science, Institute of Chemistry, P.J. Safarik University, Moyzesova 11, 04001, Kosice, Slovak Republic.

出版信息

FEMS Microbiol Lett. 2004 Jul 1;236(1):91-5. doi: 10.1016/j.femsle.2004.05.024.

DOI:10.1016/j.femsle.2004.05.024

PMID:15212796

Abstract

Analysis of restriction and modification activities in lactate-utilizing bacteria belonging to the Megasphaera elsdenii and Mitsuokella multiacida species revealed the presence of GATC-specific, MboI isospecific, restriction-modification (R-M) systems in all strains tested. While restriction endonucleases isolated from M. elsdenii strains were found to be sensitive to Dam methylation, enzymes from M. multiacida cleaved DNA irrespective of Dam methylation. The comparison of type II R-M systems specificities in three closely related lactate-utilizing ruminal bacterial species indicated complete lack of restriction and/or modification enzymes previously characterized from Selenomonas ruminantium in tested M. elsdenii and M. multiacida strains. R-M systems are believed to represent the main defense tool against phage infection. Based on the results of our experiments it could be assumed that M. elsdenii and M. multiacida use the different strategy for bacteriophage protection compared to S. ruminantium.

摘要

对属于埃氏巨球型菌和多酸 Mitsuokella 菌的利用乳酸细菌中的限制与修饰活性进行分析，结果显示，在所有测试菌株中均存在 GATC 特异性、MboI 同特异性的限制修饰（R-M）系统。虽然从埃氏巨球型菌菌株中分离出的限制性内切酶对 Dam 甲基化敏感，但多酸 Mitsuokella 菌的酶无论 Dam 甲基化情况均可切割 DNA。对三种密切相关的利用乳酸瘤胃细菌物种中的 II 型 R-M 系统特异性进行比较，结果表明，在测试的埃氏巨球型菌和多酸 Mitsuokella 菌菌株中，完全不存在先前在反刍月形单胞菌中鉴定出的限制酶和/或修饰酶。R-M 系统被认为是抵御噬菌体感染的主要防御工具。根据我们的实验结果，可以推测，与反刍月形单胞菌相比，埃氏巨球型菌和多酸 Mitsuokella 菌采用了不同的噬菌体保护策略。