瘤胃细菌的限制与修饰系统

Restriction and modification systems of ruminal bacteria.

作者信息

Pristas P, Molnárová V, Javorský P

机构信息

Institute of Animal Physiology, Slovak Academy of Sciences, 040 01 Kosice, Slovakia.

出版信息

Folia Microbiol (Praha). 2001;46(1):71-2. doi: 10.1007/BF02825890.

DOI:10.1007/BF02825890

PMID:11501482

Abstract

A high frequency of type II restriction endonuclease activities was detected in Selenomonas ruminantium but not in other rumen bacteria tested. Eight different restriction endonucleases were characterized in 17 strains coming from genetically homogeneous local population. Chromosomal DNA isolated from S. ruminantium strains was found to be refractory to cleavage by various restriction enzymes, implying the presence of methylase activities additional to those required for protection against the cellular endonucleases. The presence of Dam methylation was detected in S. ruminantium strains as well as in several other species belonging to the Sporomusa subbranch of low G + C Gram-positive bacteria (Megasphaera elsdenii, Mitsuokella multiacidus).

摘要

在反刍月形单胞菌中检测到了高频的II型限制性内切酶活性，但在测试的其他瘤胃细菌中未检测到。从遗传同质的本地群体中分离出的17株菌株中鉴定出了8种不同的限制性内切酶。发现从反刍月形单胞菌菌株中分离的染色体DNA对各种限制性酶的切割具有抗性，这意味着除了保护细胞内切酶所需的甲基化酶活性外，还存在其他甲基化酶活性。在反刍月形单胞菌菌株以及属于低G + C革兰氏阳性细菌Sporomusa亚分支的其他几个物种（埃氏巨球形菌、多酸 Mitsuokella）中检测到了Dam甲基化。