球形红杆菌AS1.1737中偶氮还原酶编码基因的表达及特性
Expression and characteristics of the gene encoding azoreductase from Rhodobacter sphaeroides AS1.1737.
作者信息
Bin Yan, Jiti Zhou, Jing Wang, Cuihong Du, Hongman Hou, Zhiyong Song, Yongming Bao
机构信息
School of Environmental and Biological Science and Technology, Dalian University of Technology, 116023, PR China.
出版信息
FEMS Microbiol Lett. 2004 Jul 1;236(1):129-36. doi: 10.1016/j.femsle.2004.05.034.
A gene that encodes a protein with azoreductase activity was obtained by PCR amplification from Rhodobacter sphaeroides AS1.1737. The enzyme, with a molecular weight of 18.7 kD, was heterologously expressed in Escherichia coli and its azoreductase activity was characterized. Furthermore, the reduction mechanism of azo dyes catalyzed by the azoreductase was studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.
通过聚合酶链反应（PCR）扩增，从球形红杆菌AS1.1737中获得了一个编码具有偶氮还原酶活性蛋白质的基因。该酶分子量为18.7 kD，在大肠杆菌中进行了异源表达，并对其偶氮还原酶活性进行了表征。此外，还详细研究了该偶氮还原酶催化偶氮染料的还原机制。鉴定出了腙中间体的存在，这为偶氮染料通过不完全还原阶段降解的假设提供了令人信服的证据。
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