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利用从新型菌株金黄色葡萄球菌KU898286中纯化得到的对氧不敏感的偶氮还原酶对偶氮活性红241进行还原。

Reduction of reactive red 241 by oxygen insensitive azoreductase purified from a novel strain Staphylococcus KU898286.

作者信息

Nisar Numrah, Aleem Amber, Saleem Faiza, Aslam Fakhra, Shahid Ammara, Chaudhry Hina, Malik Kausar, Albaser Abdulhadi, Iqbal Amjad, Qadri Rashad, Yang Yaodong

机构信息

Department of Environmental Sciences, Lahore College for Women University Lahore, Lahore, Pakistan.

Department of Biotechnology, Lahore College for Women University Lahore, Lahore, Pakistan.

出版信息

PLoS One. 2017 May 3;12(5):e0175551. doi: 10.1371/journal.pone.0175551. eCollection 2017.

DOI:10.1371/journal.pone.0175551
PMID:28467413
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5414976/
Abstract

An oxygen insensitive azoreductase was purified from a novel bacterial strain (Staphylococcus sp. KU898286) that was isolated from an abandoned site of the textile waste discharge unit. The isolated enzyme had efficiently cleaved the azo-bonds through reductive transformation under aerobic conditions. Initial phenotypic characterization and final construction of phylogenetic tree on the basis of 16s rDNA demonstrated 99% resemblance of the isolate to Staphylococcus aureus. The purified azoreductase was found to have a broad spectrum activity that reduced RR241 at a concentration of 50mg/L with pH between 6-8 and 30°C temperature). Besides, the reactive red 241 (RR241) was reduced at extracellular level as well as NADH dependent intracellular level. Complete reduction/ decolourization of RR241 were achieved after 18 hrs of exposure. The final degradation product observed to be 2-nephthol was purified by High Pressure Liquid Chromatography (HPLC) and the molecular mass was computed by Gas Chromatography-Mass spectroscopy (GC-MS). The study revealed a cost effective and eco-friendly approach to degrade the toxic dyes into less toxic products by Staphylococcus sp. KU898286.

摘要

从一个纺织废料排放单元的废弃场地分离出的新型细菌菌株（葡萄球菌属KU898286）中纯化出一种对氧气不敏感的偶氮还原酶。该分离出的酶在有氧条件下通过还原转化有效地裂解了偶氮键。基于16s rDNA的初步表型特征分析和系统发育树的最终构建表明，该分离菌株与金黄色葡萄球菌有99%的相似性。发现纯化后的偶氮还原酶具有广谱活性，在pH值为6 - 8、温度为30°C的条件下，能将浓度为50mg/L的RR241还原。此外，活性红241（RR241）在细胞外水平以及依赖于NADH的细胞内水平都能被还原。暴露18小时后，RR241实现了完全还原/脱色。通过高压液相色谱（HPLC）纯化了观察到的最终降解产物2 - 萘酚，并通过气相色谱 - 质谱联用（GC - MS）计算了其分子量。该研究揭示了一种经济有效且环保的方法，即利用葡萄球菌属KU898286将有毒染料降解为毒性较小的产物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/239e83cfafa3/pone.0175551.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/e3b2dfdc7f4c/pone.0175551.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/d6080d12336d/pone.0175551.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/b51e0acf414b/pone.0175551.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/4c44d04319bf/pone.0175551.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/27a3feb656e2/pone.0175551.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/0d431112c228/pone.0175551.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/8e4e94cd4814/pone.0175551.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/a9447e17c3b4/pone.0175551.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/239e83cfafa3/pone.0175551.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/e3b2dfdc7f4c/pone.0175551.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/d6080d12336d/pone.0175551.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/b51e0acf414b/pone.0175551.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/4c44d04319bf/pone.0175551.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/27a3feb656e2/pone.0175551.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/0d431112c228/pone.0175551.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/8e4e94cd4814/pone.0175551.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/a9447e17c3b4/pone.0175551.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffa/5414976/239e83cfafa3/pone.0175551.g009.jpg

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