Ichikawa H, Lin S-C, Tsai S Y, Tsai M-J, Sugimoto T
Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Brain Res. 2004 Jul 16;1014(1-2):247-50. doi: 10.1016/j.brainres.2004.04.024.
Immunohistochemistry for calcitonin gene-related peptide (CGRP), tyrosine hydroxylase and calbindin D-28k was performed on the glossopharyngeal and vagal ganglia in mCOUP-TFI knockout mice to know the effect of its deficiency on different types of primary sensory neurons. In wild type and heterozygous mice, the glossopharyngeal and vagal ganglia contained abundant CGRP-, tyrosine hydroxylase- and calbindin D-28k-immunoreactive (IR) neurons. In the ganglia of mCOUP-TFI knockout mice, a 38% decrease of CGRP-IR neurons was detected. However, the number of tyrosine hydroxylase- or calbindin D-28k-neurons was not altered by the mCOUP-TFI deficiency. In the tongue of knockout mice, the number of CGRP-IR nerve fibers decreased compared to wild-type and heterozygous mice. The development of CGRP-IR petrosal neurons, which supply innervation of the tongue, may depend on mCOUP-TFI.
在mCOUP-TFI基因敲除小鼠的舌咽神经节和迷走神经节上进行降钙素基因相关肽（CGRP）、酪氨酸羟化酶和钙结合蛋白D-28k的免疫组织化学检测，以了解其缺乏对不同类型初级感觉神经元的影响。在野生型和杂合子小鼠中，舌咽神经节和迷走神经节含有丰富的CGRP、酪氨酸羟化酶和钙结合蛋白D-28k免疫反应性（IR）神经元。在mCOUP-TFI基因敲除小鼠的神经节中，检测到CGRP-IR神经元减少了38%。然而，酪氨酸羟化酶或钙结合蛋白D-28k神经元的数量并未因mCOUP-TFI缺乏而改变。在基因敲除小鼠的舌头中，与野生型和杂合子小鼠相比，CGRP-IR神经纤维的数量减少。为舌头提供神经支配的CGRP-IR岩神经节神经元的发育可能依赖于mCOUP-TFI。
