Bae Nancy S, Swanson Mark J, Vassilev Alex, Howard Bruce H
Laboratory of Molecular and Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
J Biochem. 2004 Jun;135(6):695-700. doi: 10.1093/jb/mvh084.
Histone deacetylases are required for transcriptional repression in eukaryotes. Saccharomyces cerevisiae has several histone deacetylases, of which ySir2p is the most conserved throughout evolution. Currently, there is no report on the interacting protein partner of a human Sir2 homolog, SIRT2. Here we show for the first time that SIRT2 interacts with the homeobox transcription factor, HOXA10, which was identified in a two-hybrid screen. Interactions were confirmed by co-immunoprecipitation from in vitro translations as well as in human cell-free extracts. Taken together with mouse knockout studies, our results raise the intriguing possibility that SIRT2 plays a role in mammalian development.
组蛋白去乙酰化酶是真核生物转录抑制所必需的。酿酒酵母有几种组蛋白去乙酰化酶，其中ySir2p在整个进化过程中是最保守的。目前，关于人类Sir2同源物SIRT2的相互作用蛋白伙伴尚无报道。在此，我们首次表明SIRT2与同源框转录因子HOXA10相互作用，该因子是在双杂交筛选中鉴定出来的。通过体外翻译以及人无细胞提取物中的免疫共沉淀证实了这种相互作用。结合小鼠基因敲除研究，我们的结果提出了一个有趣的可能性，即SIRT2在哺乳动物发育中发挥作用。
