高效液相色谱法测定人精浆中的游离多胺

High-performance liquid chromatographic determination of free polyamines in human seminal plasma.

作者信息

Oefner P J, Wongyai S, Bonn G

机构信息

Department of Urology, Leopold-Franzens-University, Innsbruck, Austria.

出版信息

Clin Chim Acta. 1992 Jan 31;205(1-2):11-8. doi: 10.1016/0009-8981(92)90349-u.

DOI:10.1016/0009-8981(92)90349-u

PMID:1521331

Abstract

Putrescine, spermidine and spermine were determined quantitatively in 192 ultrafiltered samples of human seminal plasma with a precision of +/- 1.9% by pre-column derivatization with benzoyl chloride and reversed-phase chromatography. The concentrations of free putrescine, spermidine and spermine ranged from 0 to 1.96 mmol/l (mean = 0.10), 0.017-0.96 mmol/l (mean = 0.16) and 0.13 to 20.80 mmol/l (mean = 2.43), respectively. A significant correlation between the amounts of spermidine and spermine was found (r2 = 0.806, P less than 0.0005). Otherwise, no significant correlations between free-polyamine levels and routine spermiogram parameters such as volume, sperm count, motility, vitality and morphology were obtained.

摘要

采用苯甲酰氯柱前衍生化和反相色谱法，对192份超滤人精浆样本中的腐胺、亚精胺和精胺进行了定量测定，精密度为±1.9%。游离腐胺、亚精胺和精胺的浓度分别为0至1.96 mmol/L（平均值=0.10）、0.017至0.96 mmol/L（平均值=0.16）和0.13至20.80 mmol/L（平均值=2.43）。发现亚精胺和精胺的含量之间存在显著相关性（r2 = 0.806，P<0.0005）。否则，未获得游离多胺水平与常规精液参数（如体积、精子计数、活力、存活率和形态）之间的显著相关性。