Ikegami Takahisa, Verdier Laurent, Sakhaii Peyman, Grimme Susanne, Pescatore Barbara, Saxena Krishna, Fiebig Klaus M, Griesinger Christian
Max-Planck Institute for Biophysical Chemistry, Department of NMR Based Structural Biology, University of Frankfurt am Fassberg 11, D-37077 Göttingen, Germany.
J Biomol NMR. 2004 Jul;29(3):339-49. doi: 10.1023/B:JNMR.0000032611.72827.de.
A molecule with an anisotropic magnetic susceptibility is spontaneously aligned in a static magnetic field. Alignment of such a molecule yields residual dipolar couplings and pseudocontact shifts. Lanthanide ions have recently been successfully used to provide an anisotropic magnetic susceptibility in target molecules either by replacing a calcium ion with a lanthanide ion in calcium-binding proteins or by attaching an EDTA derivative to a cysteine residue via a disulfide bond. Here we describe a novel enantiomerically pure EDTA derived tag that aligns stronger due to its shorter linker and does not suffer from stereochemical diversity upon lanthanide complexation. We observed residual (15)N,(1)H-dipolar couplings of up to 8 Hz at 800 MHz induced by a single alignment tensor from this tag.
具有各向异性磁化率的分子会在静磁场中自发排列。这种分子的排列会产生剩余偶极耦合和赝接触位移。最近，镧系离子已成功用于在目标分子中提供各向异性磁化率，方法是在钙结合蛋白中用镧系离子取代钙离子，或者通过二硫键将EDTA衍生物连接到半胱氨酸残基上。在此，我们描述了一种新型对映体纯的EDTA衍生标签，由于其连接子较短，排列更强，并且在镧系络合时不会受到立体化学多样性的影响。我们观察到，由该标签的单个排列张量在800 MHz时诱导产生的高达8 Hz的剩余（15）N，（1）H - 偶极耦合。
