[U-14C]十六烷二酮过氧化物酶体β-氧化的中间产物。对[U-14C]十六烷二酮和[U-14C]十六烷二酮单辅酶A过氧化物酶体β-氧化过程中积累的酰基辅酶A酯的研究。

Intermediates of peroxisomal beta-oxidation of [U-14C]hexadecanedionoate. A study of the acyl-CoA esters which accumulate during peroxisomal beta-oxidation of [U-14C]hexadecanedionate and [U-14C]hexadecanedionoyl-mono-CoA.

Pourfarzam M, Bartlett K

Department of Child Health, University of Newcastle upon Tyne, England.

Eur J Biochem. 1992 Sep 1;208(2):301-7. doi: 10.1111/j.1432-1033.1992.tb17187.x.

Abstract

The oxidation of [U-14C]hexadecanedionoyl-mono-CoA was stimulated by CoA, by carnitine in the absence of CoA and by the presence of an NAD(+)-regenerating system. 2. Substrate inhibition was observed with respect to [U-14C]hexadecanedionoyl-mono-CoA at concentrations greater than 35 microM. 3. Acetyl-CoA and the dicarboxyl-CoA esters of chain length C6-16 were detected by HPLC under standard incubation conditions. 4. In the absence of the NAD(+)-regenerating system, 2-enoyl-CoA and 3-hydroxacyl-CoA esters were detected. 5. In general, the peroxisomal beta-oxidation of dicarboxylates is very similar to that of monocarboxylates [Bartlett, K., Hovik, R., Eaton, S., Watmough, N. J. & Osmundsen, H. (1990) Biochem. J. 270, 175-180] except that chain shortening does not proceed beyond C6. 6. We conclude that the peroxisomal beta-oxidation of dicarboxylates is regulated by the redox state of the peroxisomal matrix and CoA availability.

摘要

[U-14C]十六烷二酰单辅酶A的氧化受到辅酶A、在无辅酶A时肉碱以及存在NAD(+)再生系统的刺激。2. 当[U-14C]十六烷二酰单辅酶A浓度大于35微摩尔时，观察到底物抑制现象。3. 在标准孵育条件下，通过高效液相色谱法检测到乙酰辅酶A和链长为C6 - 16的二羧基辅酶A酯。4. 在无NAD(+)再生系统时，检测到2-烯酰辅酶A和3-羟酰辅酶A酯。5. 一般来说，二羧酸盐的过氧化物酶体β氧化与单羧酸盐的过氧化物酶体β氧化非常相似[巴特利特，K.，霍维克，R.，伊顿，S.，瓦特莫，N. J. & 奥斯芒森，H. (1990) 《生物化学杂志》270, 175 - 180]，只是链缩短不会超过C6。6. 我们得出结论，二羧酸盐的过氧化物酶体β氧化受限于过氧化物酶体基质的氧化还原状态和辅酶A的可用性。