可影响从氯贝丁酯处理大鼠肝脏分离的过氧化物酶体β-氧化的因素。通过垂直转子离心在自生成的Percoll梯度中分离的过氧化物酶体组分的一些特性。

Factors which can influence beta-oxidation by peroxisomes isolated from livers of clofibrate treated rats. Some properties of peroxisomal fractions isolated in a self-generated Percoll gradient by vertical rotor centrifugation.

Osmundsen H

Int J Biochem. 1982;14(10):905-14. doi: 10.1016/0020-711x(82)90074-x.

Abstract

Oxidation of radioactivity labeled acyl-CoA esters by apparently intact peroxisomes, isolated from rats treated with clofibrate, was stimulated by about 100% by added oxaloacetate. Added pyruvate gave much smaller stimulation. This stimulation is proposed to be due to re-oxidation of NADH generated during peroxisomal beta-oxidation of acyl-CoA esters. 2. Additions of these alpha-ketoacids, and of CoA, were found to influence extent of peroxisomal beta-oxidation of [U-14C]palmitoyl-CoA. Various concentrations of added CoA was markedly less inhibitory to oxidation of [14-14C]erucoyl-CoA and [10-14C]elaidoyl-CoA, as compared to [U-14C]palmitoyl-CoA. 3. Added NADP+, at concentrations higher than 0.5 mM, added NADPH and NADH were inhibitory to peroxisomal beta-oxidation of [U-14C]palmitoyl-CoA. Significant inhibition was found at concentrations which were too high for this to be of significance in vivo.

摘要

从用氯贝丁酯处理过的大鼠中分离出的明显完整的过氧化物酶体，对放射性标记的酰基辅酶A酯的氧化作用，在添加草酰乙酸后被刺激了约100%。添加丙酮酸的刺激作用则小得多。这种刺激作用被认为是由于在酰基辅酶A酯的过氧化物酶体β氧化过程中产生的NADH的再氧化。2. 发现添加这些α-酮酸以及辅酶A会影响[U-14C]棕榈酰辅酶A的过氧化物酶体β氧化程度。与[U-14C]棕榈酰辅酶A相比，各种浓度的添加辅酶A对[14-14C]芥酰辅酶A和[10-14C]反油酰辅酶A氧化的抑制作用明显较小。3. 浓度高于0.5 mM的添加NADP⁺、添加的NADPH和NADH对[U-14C]棕榈酰辅酶A的过氧化物酶体β氧化有抑制作用。在体内浓度过高而无意义的浓度下发现了显著抑制作用。