枯草芽孢杆菌168基因组重组转移的区域依赖性效率。
Region dependent efficiency for recombinational transfer of the Bacillus subtilis 168 genome.
作者信息
Tomita Satoshi, Tsuge Kenji, Kikuchi Yo, Itaya Mitsuhiro
机构信息
Division of Bioscience and Biotechnology, Department of Ecological Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan.
出版信息
Biosci Biotechnol Biochem. 2004 Jun;68(6):1382-4. doi: 10.1271/bbb.68.1382.
Submega-sized regions of the Bacillus subtilis genome were cloned to plasmid by the B. subtilis Recombinational Transfer (BReT) method. BReT efficiency depends not only on the genome location but also on the choice of sequences for simultaneous homologous recombination during BReT. In an extreme case, a 91-kb region that was unsuccessful on the first attempt was obtained when the slightly shifted 98-kb region was targeted.
通过枯草芽孢杆菌重组转移（BReT）方法将枯草芽孢杆菌基因组的超大型区域克隆到质粒中。BReT效率不仅取决于基因组位置，还取决于BReT过程中同时进行同源重组的序列选择。在一个极端情况下，当靶向稍微偏移的98 kb区域时，获得了首次尝试失败的91 kb区域。
Zotero 插件