Diderichsen B, Poulsen G B, Jørgensen S T
Novo Nordisk, Bagsvoerd, Denmark.
Plasmid. 1993 Nov;30(3):312-5. doi: 10.1006/plas.1993.1066.
We have constructed plasmid pDN1050, a new small cloning vector for Bacillus subtilis. pDN1050 harbors the origin of replication of Staphylococcus aureus plasmid pUB110 and the chloramphenicol resistance gene of S. aureus plasmid pC194. The plasmid is segregationally and structurally stable. Plasmid pDN1370, a low copy number mutant of pDN1050 was isolated and shown to harbor a mutation in the repA gene of the replication protein.
我们构建了质粒pDN1050，一种用于枯草芽孢杆菌的新型小克隆载体。pDN1050含有金黄色葡萄球菌质粒pUB110的复制起点和金黄色葡萄球菌质粒pC194的氯霉素抗性基因。该质粒在分离和结构上是稳定的。分离出了pDN1050的低拷贝数突变体质粒pDN1370，并证明其在复制蛋白的repA基因中存在突变。
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