Kawada Jun-ichi, Kimura Hiroshi, Ito Yoshinori, Hoshino Yo, Tanaka-Kitajima Naoko, Ando Yoshihiro, Futamura Masahide, Morishima Tsuneo
Department of Pediatrics, Nagoya University Graduate School of Medicine, Aichi, Japan.
Microbiol Immunol. 2004;48(5):411-5. doi: 10.1111/j.1348-0421.2004.tb03530.x.
We performed a real-time PCR assay to detect herpes simplex virus (HSV) DNA, and compared it prospectively with a nested PCR assay in 164 clinical samples (109 cerebrospinal fluid and 55 sera) from patients suspected of having neonatal HSV infection or HSV encephalitis. In 25 of 164 samples, HSV DNA was detected by the nested PCR assay. All samples positive for HSV DNA in the nested PCR assay were also positive in the real-time PCR assay, and all but two samples negative for HSV DNA in the nested assay were negative in the real-time assay. The real-time PCR assay thus had a sensitivity of 100% and a specificity of 99%, when compared with the nested assay. Sequential assays in a case of disseminated HSV showed that a decrease in HSV DNA paralleled clinical improvement. Quantification of HSV DNA by real-time PCR was useful for diagnosing and monitoring patients with HSV encephalitis and neonatal HSV infection.
我们进行了实时聚合酶链反应（PCR）检测单纯疱疹病毒（HSV）DNA，并将其与巢式PCR检测法进行前瞻性比较，检测了164份疑似患有新生儿HSV感染或HSV脑炎患者的临床样本（109份脑脊液和55份血清）。在164份样本中的25份中，巢式PCR检测法检测到了HSV DNA。巢式PCR检测法中所有HSV DNA阳性的样本在实时PCR检测中也呈阳性，巢式检测法中除两份样本外所有HSV DNA阴性的样本在实时检测中也呈阴性。因此，与巢式检测法相比，实时PCR检测法的灵敏度为100%，特异性为99%。对一例播散性HSV病例进行的连续检测显示，HSV DNA的减少与临床改善情况平行。通过实时PCR对HSV DNA进行定量分析，有助于诊断和监测HSV脑炎及新生儿HSV感染患者。
