Rittig M, Lütjen-Drecoll E, Prehm P
Department of Anatomy, University of Erlangen-Nürnberg, Germany.
Exp Eye Res. 1992 Mar;54(3):455-60. doi: 10.1016/0014-4835(92)90057-y.
Distribution of hyaluronan synthase was investigated in cornea and conjunctiva of Cynomolgus monkeys (Macaca fascicularis) using polyclonal antibodies against the streptococcal enzyme. Strong immunoreaction was found in the cell membranes of the corneal endothelium, corneal epithelium, and most of the conjunctival epithelium. In the corneal epithelium all cells except the basal ones stained. In the conjunctiva all cylindrical cells stained, whereas among the goblet cells one type showed intense membrane staining, the other remained unstained. In the limbal portion of the conjunctival epithelium, which in many other respects differs morphologically and functionally from the remaining conjunctiva, all membranes of the different layers of the stratified epithelium except the most superficial ones, appeared unstained. Staining was also seen in all stromal fibroblasts and capillary endothelial cells.
使用抗链球菌酶的多克隆抗体，研究了食蟹猴（猕猴属）角膜和结膜中透明质酸合酶的分布。在角膜内皮、角膜上皮和大部分结膜上皮的细胞膜中发现了强烈的免疫反应。在角膜上皮中，除基底细胞外所有细胞均被染色。在结膜中，所有柱状细胞均被染色，而杯状细胞中一种类型显示强烈的膜染色，另一种则未染色。在结膜上皮的角膜缘部分，其在许多其他方面在形态和功能上与其余结膜不同，分层上皮不同层的所有膜除最表层外均未染色。在所有基质成纤维细胞和毛细血管内皮细胞中也可见染色。
