Brzezinski A, Fibich T, Cohen M, Schenker J G, Laufer N
Department of Obstetrics and Gynecology, Hadassah Medical Center, Jerusalem, Israel.
Fertil Steril. 1992 Sep;58(3):526-9. doi: 10.1016/s0015-0282(16)55257-1.
To test the hypothesis that melatonin modulates steroid synthesis in the human ovary.
Granulosa lutein cells obtained from in vitro fertilization cycles were cultured in medium containing melatonin and human chorionic gonadotropin (hCG).
Progesterone (P) secretion by granulosa lutein cells increased progressively in both basal and hCG-stimulated conditions, up to 96 hours in culture, plateaued at 144 and decreased thereafter. Melatonin (10(-7), 10(-9), 10(-11) M) had no effect on basal P or 17 beta-estradiol production. The addition of melatonin to the hCG-treated granulosa lutein cells significantly (P less than 0.05) potentiated the stimulatory effect of hCG on P production. The effect was most prominent after 144 and 196 hours of incubation.
This observation suggests a role for melatonin in the intraovarian control of P production in the human ovary.
验证褪黑素调节人类卵巢甾体激素合成这一假说。
从体外受精周期获取的颗粒黄体细胞在含有褪黑素和人绒毛膜促性腺激素（hCG）的培养基中培养。
在基础条件和hCG刺激条件下，颗粒黄体细胞分泌的孕酮（P）均逐渐增加，培养至96小时达到峰值，144小时时趋于平稳，之后下降。褪黑素（10⁻⁷、10⁻⁹、10⁻¹¹ M）对基础P或17β-雌二醇的产生无影响。在hCG处理的颗粒黄体细胞中添加褪黑素可显著（P<0.05）增强hCG对P产生的刺激作用。在孵育144小时和196小时后，该作用最为显著。
这一观察结果提示褪黑素在人类卵巢内P产生的调控中发挥作用。
