从海参中分离出的纹孢枝顶孢真菌的新糖苷。

New glycosides of the fungus Acremonium striatisporum isolated from a sea cucumber.

作者信息

Afiyatullov Shamil Sh, Kalinovsky Anatoly I, Kuznetsova Tatyana A, Pivkin Mikhail V, Prokof'eva Nina G, Dmitrenok Pavel S, Elyakov George B

机构信息

Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok 22, Russian Federation.

出版信息

J Nat Prod. 2004 Jun;67(6):1047-51. doi: 10.1021/np0305324.

DOI:10.1021/np0305324

PMID:15217294

Abstract

Four new diterpene glycosides, virescenosides R (1), S (2), T (3), and U (4), have been isolated from a marine strain of Acremonium striatisporum KMM 4401 associated with the holothurian Eupentacta fraudatrix. Their structures have been elucidated on the basis of HRFABMS, 1D and 2D NMR (1H, 13C, DEPT, COSY-45, COSY-RCT, HSQC, HMBC, and NOESY spectra), and the results of acidic hydrolysis as 19-O-[beta-D-glucopyranosyl(1-->6)-beta-d-altropyranosyl]-isopimara-7,15-diene-2alpha,3beta-diol (1), 19-O-beta-D-altropyranosyl-3-oxo-isopimara-8(14),15-diene-7alpha-ol (2), 19-O-beta-D-altropyranosyl-3,7-dioxo-isopimara-8,15-diene (3), and 19-O-beta-D-altropyranosyl-3,7-dioxo-isopimara-8(14),15-diene (4). The cytotoxic activity of the virescenosides was examined.

摘要

从与海参Eupentacta fraudatrix相关的海洋枝顶孢菌Acremonium striatisporum KMM 4401菌株中分离出四种新的二萜糖苷，即绿菌素R（1）、S（2）、T（3）和U（4）。基于高分辨快原子轰击质谱（HRFABMS）、一维和二维核磁共振（1H、13C、DEPT、COSY - 45、COSY - RCT、HSQC、HMBC和NOESY谱）以及酸性水解结果，确定了它们的结构，分别为19 - O - [β - D - 吡喃葡萄糖基(1→6) - β - D - 阿卓吡喃糖基] - 异海松 - 7,15 - 二烯 - 2α,3β - 二醇（1）、19 - O - β - D - 阿卓吡喃糖基 - 3 - 氧代 - 异海松 - 8(14),15 - 二烯 - 7α - 醇（2）、19 - O - β - D - 阿卓吡喃糖基 - 3,7 - 二氧代 - 异海松 - 8,15 - 二烯（3）和19 - O - β - D - 阿卓吡喃糖基 - 3,7 - 二氧代 - 异海松 - 8(14),15 - 二烯（4）。检测了绿菌素的细胞毒性活性。