从活性污泥中分离出的不动杆菌属细菌中多聚磷酸盐和聚-β-羟基丁酸酯颗粒的鉴别
Differentiation of polyphosphate and poly-beta-hydroxybutyrate granules in an Acinetobacter sp. isolated from activated sludge.
作者信息
Rees G N, Vasiliadis G, May J W, Bayly R C
机构信息
Department of Microbiology, Monash University, Clayton, Victoria, Australia.
出版信息
FEMS Microbiol Lett. 1992 Jul 1;73(1-2):171-3. doi: 10.1016/0378-1097(92)90603-l.
Cells containing polyphosphate 71 micrograms P (mg protein)-1 and no poly-beta-hydroxybutyrate showed metachromatic granules but no lipid granules; cells containing poly-beta-hydroxybutyrate (15% of dry weight) showed fluorescence lipid granules but no metachromatic granules; whereas cells containing both polyphosphate and poly-beta-hydroxybutyrate showed both types of granules. These observations, together with a critical review of the literature, show a clear distinction between metachromatic (or volutin) granules and lipid granules.
含有71微克磷（毫克蛋白）⁻¹的多聚磷酸盐且不含聚-β-羟基丁酸酯的细胞呈现异染粒但无脂粒；含有聚-β-羟基丁酸酯（干重的15%）的细胞呈现荧光脂粒但无异染粒；而同时含有多聚磷酸盐和聚-β-羟基丁酸酯的细胞则呈现两种颗粒。这些观察结果，连同对文献的批判性综述，表明异染（或迂回体）颗粒和脂粒之间有明显区别。
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