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从活性污泥中分离出的不动杆菌属细菌中多聚磷酸盐和聚-β-羟基丁酸酯颗粒的鉴别

Differentiation of polyphosphate and poly-beta-hydroxybutyrate granules in an Acinetobacter sp. isolated from activated sludge.

作者信息

Rees G N, Vasiliadis G, May J W, Bayly R C

机构信息

Department of Microbiology, Monash University, Clayton, Victoria, Australia.

出版信息

FEMS Microbiol Lett. 1992 Jul 1;73(1-2):171-3. doi: 10.1016/0378-1097(92)90603-l.

Abstract

Cells containing polyphosphate 71 micrograms P (mg protein)-1 and no poly-beta-hydroxybutyrate showed metachromatic granules but no lipid granules; cells containing poly-beta-hydroxybutyrate (15% of dry weight) showed fluorescence lipid granules but no metachromatic granules; whereas cells containing both polyphosphate and poly-beta-hydroxybutyrate showed both types of granules. These observations, together with a critical review of the literature, show a clear distinction between metachromatic (or volutin) granules and lipid granules.

摘要

含有71微克磷(毫克蛋白)⁻¹的多聚磷酸盐且不含聚-β-羟基丁酸酯的细胞呈现异染粒但无脂粒;含有聚-β-羟基丁酸酯(干重的15%)的细胞呈现荧光脂粒但无异染粒;而同时含有多聚磷酸盐和聚-β-羟基丁酸酯的细胞则呈现两种颗粒。这些观察结果,连同对文献的批判性综述,表明异染(或迂回体)颗粒和脂粒之间有明显区别。

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