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尼罗蓝A作为聚β-羟基丁酸酯的荧光染色剂。

Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate.

作者信息

Ostle A G, Holt J G

出版信息

Appl Environ Microbiol. 1982 Jul;44(1):238-41. doi: 10.1128/aem.44.1.238-241.1982.

DOI:10.1128/aem.44.1.238-241.1982
PMID:6181737
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC241995/
Abstract

Poly-beta-hydroxybutyrate granules exhibited a strong orange fluorescence when stained with Nile blue A. Heat-fixed cells were treated with 1% Nile blue A for 10 min and were observed at an excitation wavelength of 460 nm. Glycogen and polyphosphate did not stain. Nile blue A appears to be a more specific stain for poly-beta-hydroxybutyrate than Sudan black B.

摘要

用尼罗蓝A染色时，聚-β-羟基丁酸酯颗粒呈现出强烈的橙色荧光。将热固定的细胞用1%尼罗蓝A处理10分钟，并在460nm的激发波长下观察。糖原和多磷酸盐未被染色。尼罗蓝A似乎是一种比苏丹黑B对聚-β-羟基丁酸酯更具特异性的染色剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7735/241995/ab5924a95195/aem00176-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7735/241995/dc2495ae9cb7/aem00176-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7735/241995/ab5924a95195/aem00176-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7735/241995/dc2495ae9cb7/aem00176-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7735/241995/ab5924a95195/aem00176-0247-a.jpg

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