不断扩大的细菌群体的pH值变化轨迹。
pH track of expanding bacterial populations.
作者信息
Reshetilov A N, Medvinsky A B, Eliseeva T P, Tsyganov M A, Boronin A M, Ivanitsky G R
机构信息
Institute of Radioengineering, Electronics and Automation of Moscow, Russia.
出版信息
FEMS Microbiol Lett. 1992 Jul 1;73(1-2):59-62. doi: 10.1016/0378-1097(92)90583-a.
A method of pH distribution measurements in agar nutrient media containing expanding bacterial populations is described. It is based on measuring pH microsamples taken at different points of the media. The sample volume was 10 microliters. A pH sensitive field effect transistor was used as a measuring electrode. Acidification was found to occur in glucose media, while alkalization occurred in the media containing peptone.
描述了一种在含有不断扩展细菌群体的琼脂营养培养基中进行pH分布测量的方法。该方法基于测量在培养基不同点采集的pH微样本。样本体积为10微升。使用pH敏感场效应晶体管作为测量电极。发现葡萄糖培养基中发生酸化，而含有蛋白胨的培养基中发生碱化。
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