大肠杆菌在富集培养基中产气的变异性及其与pH的关系。
Variability in gas production by Escherichia coli in enrichment media and its relationship to pH.
作者信息
Meadows P S, Anderson J G, Patel K, Mullins B W
出版信息
Appl Environ Microbiol. 1980 Aug;40(2):309-12. doi: 10.1128/aem.40.2.309-312.1980.
Variability in gas production in multiple subcultures of Escherichia coli was assessed in two selective enrichment media and in lactose peptone water. Considerable variability occurred with all media at 37 and 44 degrees C. Addition of buffer increased gas production and decreased variability. The relationships between pH, growth, and gas production were complex. In buffered media, viable counts increased by 269 x 10(6) to 382 x 10(6)/U of pH fall, whereas in unbuffered media, they increased by 9.45 x 10(6) to 30.37 x 10(6)/U of pH fall. In buffered and unbuffered media, pH fell as gas production rose. However, variability in gas production among individual subcultures was not associated with changes in pH.
在两种选择性增菌培养基和乳糖蛋白胨水中评估了大肠杆菌多个继代培养物中产气的变异性。在37℃和44℃下，所有培养基均出现了相当大的变异性。添加缓冲液可增加产气并降低变异性。pH、生长和产气之间的关系很复杂。在缓冲培养基中，每单位pH下降，活菌数增加269×10⁶至382×10⁶个，而在未缓冲培养基中，每单位pH下降，活菌数增加9.45×10⁶至30.37×10⁶个。在缓冲和未缓冲培养基中，产气增加时pH下降。然而，各个继代培养物之间产气的变异性与pH的变化无关。
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