Henrichsen J, Robbins J B
WHO Collaborating Centre for Reference and Research on Pneumococci, Statens Seruminstitut, Copenhagen, Denmark.
FEMS Microbiol Lett. 1992 Jul 1;73(1-2):89-93. doi: 10.1016/0378-1097(92)90589-g.
Hyperimmune and high-titered polyclonal pneumococcal antisera, specific for cross-reactive types within groups, were produced in adult rabbits. Purified capsular polysaccharide was injected intravenously into adult rabbits. One week later, these rabbits were given multiple intravenous injections of formalin-inactivated pneumococci of the cross-reactive type by an established method. Each of the resultant antisera were specific for the cross-reactive type indicating that the previous injection of the polysaccharide had induced epitope-specific tolerance. This method was successful for production of antisera against pneumococcal types 6A, 6B, 9N, 9V, 19F and 19A. Polyclonal rabbit pneumococcal antisera have some advantages over murine monoclonal antibodies for serologic studies and this method should be applicable for producing type-specific antibodies to cross-reactive polysaccharides of clinical interest. Further, this method is simpler and generally produces higher titered monovalent (factor) reagents than absorbed antisera.
针对各血清群内交叉反应型别的超免疫和高滴度多克隆肺炎球菌抗血清是在成年兔体内制备的。将纯化的荚膜多糖静脉注射到成年兔体内。一周后，通过既定方法给这些兔子多次静脉注射经福尔马林灭活的交叉反应型肺炎球菌。所得到的每种抗血清都对交叉反应型别具有特异性，这表明先前注射多糖已诱导了表位特异性耐受。该方法成功制备出了针对6A、6B、9N、9V、19F和19A血清型肺炎球菌的抗血清。多克隆兔肺炎球菌抗血清在血清学研究中比鼠单克隆抗体具有一些优势，并且该方法应适用于生产针对具有临床意义的交叉反应多糖的型特异性抗体。此外，该方法更简单，并且通常比吸收后的抗血清产生更高滴度的单价（因子）试剂。
