Hui Eric Ka-Wai, Yap Ee Ming, An Dong Sung, Chen Irvin S Y, Nayak Debi P
Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
Departments of Microbiology, Immunology, and Molecular Genetics and Medicine, UCLA AIDS Institute, Los Angeles, CA 90095, USA.
J Gen Virol. 2004 Jul;85(Pt 7):1877-1884. doi: 10.1099/vir.0.79906-0.
Small interfering RNA (siRNA)-induced RNA degradation has been used recently as an antivirus agent to inhibit specific virus replication. This report shows that 21 nt duplexes of siRNA of the influenza virus M gene can cause specific inhibition of influenza virus matrix (M1) protein expression in transfected 293T cells. Furthermore, it is shown that a lentivirus vector can be used to effectively deliver M gene siRNAs into Madin-Darby canine kidney cells and can cause specific inhibition of M1 protein expression and influenza virus replication. Therefore, lentivirus-mediated delivery of siRNA and gene silencing can be used in studying the specific functions of virus genes in replication and may have a potential therapeutic application.
小干扰RNA（siRNA）诱导的RNA降解最近已被用作抗病毒剂来抑制特定病毒的复制。本报告表明，流感病毒M基因的21个核苷酸双链体siRNA可在转染的293T细胞中特异性抑制流感病毒基质（M1）蛋白的表达。此外，研究表明慢病毒载体可有效将M基因siRNAs导入犬肾传代细胞（Madin-Darby canine kidney cells），并可特异性抑制M1蛋白表达和流感病毒复制。因此，慢病毒介导的siRNA递送和基因沉默可用于研究病毒基因在复制中的特定功能，并可能具有潜在的治疗应用价值。
