Ocalewicz K, Sliwinska A, Jankun M
Department of Evolutionary Genetics, University of Warmia and Mazury, Olsztyn, Poland.
Cytogenet Genome Res. 2004;105(1):79-82. doi: 10.1159/000078012.
Cytogenetic analysis of brook trout performed with molecular and conventional methods led to identification of interstitial telomeric sites on one or two subtelocentric chromosomes within the same pair. Morphology and specific patterns of these chromosomes using fluorochromes associated with A/T- or G/C-rich DNA proved that these chromosomes are not sex related. The chromomycin-positive region was located on the short arms of the ITS bearing chromosome pair and flanked by telomeric sequences, suggesting that this part of the chromosome had been translocated from another one. Our observations confirm that GC-rich regions are highly mobile genetic structures, and led to ITS formation on brook trout chromosomes.
采用分子和传统方法对溪红点鲑进行细胞遗传学分析，结果在同一对中的一条或两条亚近端着丝粒染色体上鉴定出了中间端粒位点。使用与富含A/T或G/C的DNA相关的荧光染料对这些染色体的形态和特定模式进行分析，结果证明这些染色体与性别无关。嗜铬霉素阳性区域位于携带中间端粒序列（ITS）的染色体对的短臂上，并由端粒序列侧翼包围，这表明该染色体的这一部分是从另一条染色体易位而来的。我们的观察结果证实，富含GC的区域是高度可移动的遗传结构，并导致溪红点鲑染色体上形成了中间端粒序列。
