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鲑科鱼类的染色体显带:鲑属和红点鲑属中的核仁组织区

Chromosome banding in salmonid fish: nucleolar organizer regions in Salmo and Salvelinus.

作者信息

Phillips R, Ihssen P E

出版信息

Can J Genet Cytol. 1985 Aug;27(4):433-40. doi: 10.1139/g85-064.

DOI:10.1139/g85-064
PMID:4027775
Abstract

Chromosome banding patterns obtained by silver staining (Ag-NORs) were analyzed in three species of Salmo (rainbow, brown trout, and Atlantic salmon) and three species of Salvelinus (brook trout, lake trout, and arctic char). In rainbow trout and Atlantic salmon the Ag-NORs were found at the secondary constrictions of a single chromosome pair, while in brown trout the Ag-NORs were found on the short arms of one or two of the two longest subtelocentric or acrocentric chromosome pairs. The location of the Ag-NORs was multichromosomal in the three Salvelinus species, occurring on one or both members of four to six different chromosome pairs in different individuals. The Ag-NOR sites were on the short arms of some acrocentric pairs and at the telomeres of other acrocentric pairs and one or two metacentric pairs. Chromomycin A3 positive bands were found at the same sites as the Ag-NORs in all species. In the species with multichromosomal location of Ag-NORs, polymorphisms in the size and location of the NORs were extremely common, so that almost every individual fish had a different pattern of Ag-NOR sites.

摘要

对三种鲑属鱼类(虹鳟、褐鳟和大西洋鲑)和三种红点鲑属鱼类(溪红点鲑、湖红点鲑和北极红点鲑)通过银染(Ag-NORs)获得的染色体带型进行了分析。在虹鳟和大西洋鲑中,Ag-NORs位于一对单一染色体的次缢痕处,而在褐鳟中,Ag-NORs位于两个最长的亚端着丝粒或近端着丝粒染色体对中一个或两个的短臂上。Ag-NORs的位置在三种红点鲑属鱼类中是多染色体的,在不同个体中出现在四到六个不同染色体对的一个或两个成员上。Ag-NOR位点位于一些近端着丝粒对的短臂上以及其他近端着丝粒对和一到两个中着丝粒对的端粒处。在所有物种中,发现放线菌素A3阳性带与Ag-NORs位于相同位置。在Ag-NORs多染色体定位的物种中,NORs大小和位置的多态性极为常见,以至于几乎每条个体鱼都有不同的Ag-NOR位点模式。

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