Seol Yang-Jo, Lee Jue-Yeon, Park Yoon-Jeong, Lee Yong-Moo, Rhyu In-Chul, Lee Seung-Jin, Han Soo-Boo, Chung Chong-Pyoung
Department of Periodontology, College of Dentistry, Seoul National University, 28-2 Yongon-Dong, Chongno-Ku, Seoul 110-749, South Korea.
Biotechnol Lett. 2004 Jul;26(13):1037-41. doi: 10.1023/B:BILE.0000032962.79531.fd.
Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 microm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 x 10(6) (day 1), 11 x 10(6) (day 28) and 12 x 10(6) (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) microg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.
将大鼠颅骨成骨细胞培养于通过冷冻干燥制备的多孔壳聚糖海绵中。所制备的壳聚糖海绵具有孔径为100 - 200微米的多孔结构，这有利于细胞增殖。在长达56天的培养过程中监测细胞密度、碱性磷酸酶活性和钙沉积情况。每克海绵中的细胞数量在第1天为4×10⁶、第28天为11×10⁶、第56天为12×10⁶。每块海绵的钙沉积量在第1天为9微克、第28天为40微克、第56天为48微克。组织学结果证实海绵内部已发生骨形成。这些结果表明壳聚糖海绵可作为体外组织工程骨形成的有效支架材料。
