Chonanant Chirapond, Chancharoen Pongrung, Kiatkulanusorn Sirirat, Luangpon Nongnuch, Klarod Kultida, Surakul Pornprom, Thamwiriyasati Niramon, Singsanan Sanita, Ngernyuang Nipaporn
Department of Medical Technology, Faculty of Allied Health Science, Burapha University, Chonburi, 20131, Thailand.
Department of Medical Sciences, Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand.
ACS Omega. 2024 Sep 10;9(38):39419-39429. doi: 10.1021/acsomega.4c02910. eCollection 2024 Sep 24.
Chitosan-based scaffolding possesses unique properties that make it highly suitable for tissue engineering applications. Chitosan is derived from deacetylating chitin, which is particularly abundant in the shells of crustaceans. This study aimed to extract chitosan from shrimp shell waste () and produce biocomposite scaffolds using the extracted chitosan for cartilage tissue engineering applications. Chitinous material from shrimp shell waste was deproteinized and deacetylated. The extracted chitosan was characterized and compared to commercial chitosan through various physicochemical analyses. The findings revealed that the extracted chitosan shares similar trends in the Fourier transform infrared spectroscopy spectrum, energy dispersive X-ray mapping, and X-ray diffraction pattern to commercial chitosan. Despite differences in the degree of deacetylation, these results underscore its comparable quality. The extracted chitosan was mixed with agarose, collagen, and gelatin to produce the blending biocomposite AG-CH-COL-GEL scaffold by freeze-drying method. Results showed AG-CH-COL-GEL scaffolds have a 3D interconnected porous structure with pore size 88-278 μm, high water uptake capacity (>90%), and degradation percentages in 21 days between 5.08% and 30.29%. Mechanical compression testing revealed that the elastic modulus of AG-CH-COL-GEL scaffolds ranged from 44.91 to 201.77 KPa. Moreover, AG-CH-COL-GEL scaffolds have shown significant potential in effectively inducing human chondrocyte proliferation and enhancing aggrecan gene expression. In conclusion, AG-CH-COL-GEL scaffolds emerge as promising candidates for cartilage tissue engineering with their optimal physical properties and excellent biocompatibility. This study highlights the potential of using waste-derived chitosan and opens new avenues for sustainable and effective tissue engineering solutions
基于壳聚糖的支架具有独特的性能，使其非常适合组织工程应用。壳聚糖是由甲壳素脱乙酰化而来，甲壳素在甲壳类动物的外壳中含量特别丰富。本研究旨在从虾壳废料中提取壳聚糖，并使用提取的壳聚糖制备生物复合支架用于软骨组织工程应用。对虾壳废料中的几丁质材料进行了脱蛋白和脱乙酰化处理。对提取的壳聚糖进行了表征，并通过各种物理化学分析与商业壳聚糖进行了比较。研究结果表明，提取的壳聚糖在傅里叶变换红外光谱、能量色散X射线图谱和X射线衍射图谱方面与商业壳聚糖具有相似的趋势。尽管脱乙酰度存在差异，但这些结果强调了其质量的可比性。将提取的壳聚糖与琼脂糖、胶原蛋白和明胶混合，通过冷冻干燥法制备了共混生物复合AG-CH-COL-GEL支架。结果表明，AG-CH-COL-GEL支架具有三维相互连接的多孔结构，孔径为88-278μm，高吸水能力（>90%），21天内降解率在5.08%至30.29%之间。机械压缩测试表明，AG-CH-COL-GEL支架的弹性模量在44.91至201.77 KPa之间。此外，AG-CH-COL-GEL支架在有效诱导人软骨细胞增殖和增强聚集蛋白聚糖基因表达方面显示出巨大潜力。总之，AG-CH-COL-GEL支架凭借其最佳的物理性能和优异的生物相容性，成为软骨组织工程的有前途的候选材料。本研究突出了利用废弃物衍生壳聚糖的潜力，并为可持续和有效的组织工程解决方案开辟了新途径。
