固定化南极假丝酵母脂肪酶在减压条件下催化黄酮类化合物的区域选择性酰化反应。

Regioselective acylation of flavonoids catalyzed by immobilized Candida antarctica lipase under reduced pressure.

作者信息

Passicos Emilie, Santarelli Xavier, Coulon Denis

机构信息

Ecole Supérieure de Technologie des Biomolécules de Bordeaux, Université Victor Segalen Bordeaux2, 146 rue Léo Saignat, 33076 Bordeaux cedex, France.

出版信息

Biotechnol Lett. 2004 Jul;26(13):1073-6. doi: 10.1023/B:BILE.0000032967.23282.15.

DOI:10.1023/B:BILE.0000032967.23282.15

PMID:15218382

Abstract

A single-step acylation of rutin and naringin, catalyzed by immobilized Candida antarctica lipase B in 2-methyl-2-butanol, occurred preferentially on the primary hydroxyl group. Using palmitic methyl ester as acyl donor, the acylation rate of naringin was 10-fold higher than that of rutin. Under optimal conditions, i.e. a molar ratio acyl donor/naringin of 7:1 and 200 mbar, 92% naringin was acylated.

摘要

在2-甲基-2-丁醇中，固定化的南极假丝酵母脂肪酶B催化芦丁和柚皮苷的单步酰化反应，优先发生在伯羟基上。以棕榈酸甲酯作为酰基供体，柚皮苷的酰化速率比芦丁高10倍。在最佳条件下，即酰基供体与柚皮苷的摩尔比为7:1且压力为200毫巴时，92%的柚皮苷被酰化。