Passicos Emilie, Santarelli Xavier, Coulon Denis
Ecole Supérieure de Technologie des Biomolécules de Bordeaux, Université Victor Segalen Bordeaux2, 146 rue Léo Saignat, 33076 Bordeaux cedex, France.
Biotechnol Lett. 2004 Jul;26(13):1073-6. doi: 10.1023/B:BILE.0000032967.23282.15.
A single-step acylation of rutin and naringin, catalyzed by immobilized Candida antarctica lipase B in 2-methyl-2-butanol, occurred preferentially on the primary hydroxyl group. Using palmitic methyl ester as acyl donor, the acylation rate of naringin was 10-fold higher than that of rutin. Under optimal conditions, i.e. a molar ratio acyl donor/naringin of 7:1 and 200 mbar, 92% naringin was acylated.
在2-甲基-2-丁醇中，固定化的南极假丝酵母脂肪酶B催化芦丁和柚皮苷的单步酰化反应，优先发生在伯羟基上。以棕榈酸甲酯作为酰基供体，柚皮苷的酰化速率比芦丁高10倍。在最佳条件下，即酰基供体与柚皮苷的摩尔比为7:1且压力为200毫巴时，92%的柚皮苷被酰化。
