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波兰养殖水貂的弓形虫感染

Toxoplasma gondii infection in Polish farmed mink.

作者信息

Smielewska-Łoś Ewa, Turniak Waldemar

机构信息

Department of Epizootiology and Veterinary Administration with Clinic, Faculty of Veterinary Medicine, Agricultural University, pl. Grunwaldzki 45, 50-366 Wroclaw, Poland.

出版信息

Vet Parasitol. 2004 Jul 14;122(3):201-6. doi: 10.1016/j.vetpar.2004.02.003.

Abstract

The aim of this study was to determine the seroprevalence of Toxoplasma gondii antibodies in Polish farmed mink according to way of feeding as well as to confirm the role of toxoplasmosis in reproductive losses in mink farms. The serological examinations were carried out on 961 mink randomly selected from 12 Polish farms. Blood sera were examined for the presence of T. gondii antibodies with the use of the latex agglutination test. The examinations for the presence of T. gondii in organ tissues were performed on five neonatal mink kits with the use of immunofluorescence method. In total 133 (13.9%) out of 961 examined mink had T. gondii antibodies. In large farms the seropositivity was lower (2.9%), than in small farms (26.33%) (P < 0.001). Significant difference was found in seroprevalence according to way of feeding. In farms feeding fish, percentage of seropositivity was lower (2.2%), than in farms based on non-frozen slaughter offal (43.4%). Titres of T. gondii antibodies were usually lower than 120 IU/ml. Using the immunofluorescence method, T. gondii was detected in impression smears from liver and brain of two neonatal mink kits derived from one seropositive female.

摘要

本研究的目的是根据饲养方式确定波兰养殖水貂中弓形虫抗体的血清流行率,并证实弓形虫病在水貂养殖场繁殖损失中的作用。对从12个波兰养殖场随机选取的961只水貂进行了血清学检查。使用乳胶凝集试验检测血清中是否存在弓形虫抗体。使用免疫荧光法对5只新生水貂幼崽的器官组织进行了弓形虫检测。在961只接受检查的水貂中,共有133只(13.9%)含有弓形虫抗体。大型养殖场的血清阳性率(2.9%)低于小型养殖场(26.33%)(P<0.001)。根据饲养方式,血清流行率存在显著差异。在投喂鱼类的养殖场,血清阳性率(2.2%)低于以非冷冻屠宰下脚料为食的养殖场(43.4%)。弓形虫抗体滴度通常低于120 IU/ml。使用免疫荧光法,在来自一只血清阳性雌性的两只新生水貂幼崽的肝脏和脑印片中检测到了弓形虫。

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