中国北方养殖水貂（Neovison vison）中弓形虫的PCR-RFLP分子检测及遗传特征分析
Molecular Detection and Genetic Characterization of Toxoplasma gondii in Farmed Minks (Neovison vison) in Northern China by PCR-RFLP.
作者信息
Zheng Wen-Bin, Zhang Xiao-Xuan, Ma Jian-Gang, Li Fa-Cai, Zhao Quan, Huang Si-Yang, Zhu Xing-Quan
机构信息
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, PR China.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province, PR China.
出版信息
PLoS One. 2016 Nov 2;11(11):e0165308. doi: 10.1371/journal.pone.0165308. eCollection 2016.
Toxoplasma gondii is a worldwide prevalent parasite, affecting a wide range of mammals and human beings. Little information is available about the distribution of genetic diversity of T. gondii infection in minks (Neovison vison). This study was conducted to estimate the prevalence and genetic characterization of T. gondii isolates from minks in China. A total of 418 minks brain tissue samples were collected from Jilin and Hebei provinces, northern China. Genomic DNA were extracted and assayed for T. gondii infection by semi-nested PCR of B1 gene. The positive DNA samples were typed at 10 genetic markers (SAG1, SAG2 (5'+3' SAG2, alter.SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. 36 (8.6%) of 418 DNA samples were overall positive for T. gondii. Among them, 5 samples were genotyped at all loci, and 1 sample was genotyped for 9 loci. In total, five samples belong to ToxoDB PCR-RFLP genotype#9, one belong to ToxoDB genotye#3. To our knowledge, this is the first report of genetic characterization of T. gondii in minks in China. Meanwhile, these results revealed a distribution of T. gondii infection in minks in China. These data provided base-line information for controlling T. gondii infection in minks.
刚地弓形虫是一种在全球广泛流行的寄生虫，可感染多种哺乳动物和人类。关于水貂（Neovison vison）中刚地弓形虫感染的遗传多样性分布，目前所知甚少。本研究旨在评估中国水貂中刚地弓形虫分离株的感染率及其遗传特征。从中国北方的吉林省和河北省共采集了418份水貂脑组织样本。提取基因组DNA，并通过B1基因的半巢式PCR检测刚地弓形虫感染情况。对阳性DNA样本使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，在10个遗传标记（SAG1、SAG2（5'+3' SAG2、alter.SAG2）、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Apico）上进行分型。418份DNA样本中，共有36份（8.6%）总体呈刚地弓形虫阳性。其中，5份样本在所有位点均被分型，1份样本在9个位点被分型。总共5份样本属于ToxoDB PCR-RFLP基因型#9，1份属于ToxoDB基因型#3。据我们所知，这是中国水貂中刚地弓形虫遗传特征的首次报道。同时，这些结果揭示了中国水貂中刚地弓形虫感染的分布情况。这些数据为控制水貂中的刚地弓形虫感染提供了基线信息。
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