HLA-A和-B等位基因表达以及C亚型HIV-1感染的埃塞俄比亚人产生抗Gag跨亚型反应的能力。

HLA-A and -B allele expression and ability to develop anti-Gag cross-clade responses in subtype C HIV-1-infected Ethiopians.

作者信息

Ferrari Guido, Currier Jeffrey R, Harris Matthew E, Finkelstein Stella, de Oliveira Angelica, Barkhan Debra, Cox Josephine H, Zeira Michael, Weinhold Kent J, Reinsmoen Nancy, McCutchan Francine, Birx Deborah L, Osmanov Saladin, Maayan Shlomo

机构信息

Duke University, Durham, NC, USA.

出版信息

Hum Immunol. 2004 Jun;65(6):648-59. doi: 10.1016/j.humimm.2004.02.031.

DOI:10.1016/j.humimm.2004.02.031

PMID:15219385

Abstract

A cohort of 35 human immunodeficiency virus type 1 (HIV-1) subtype C-infected Ethiopians was studied to define the HLA phenotype in all 35 subjects and highly conserved Gag protein regions involved in cross-clade cell-mediated immunity. Full-length Gag virus sequences were determined in 15 individuals. CD8 cell-mediated immune responses were detected by interferon-gamma ELISpot assay. HLA-A03, -B49, and -B57 allelic frequencies were relatively higher than in other African populations. Anti-p17 (aa 1-60) CD8+ were detectable in the highest number of individuals. Anti-p17 (aa 1-60 and 51-110) cross-clade responses against subtype B and C were detected in 50% of the tested subjects. The p24 KF11 (aa 162-172) epitope was found to be immunodominant among the HLA-B5703--positive individuals. These data represent the first report of correlating HLA phenotype and HIV-specific cell-mediated immune responses among infected Ethiopians and may be useful in designing cytotoxic T lymphocyte-inducing vaccines for this part of Africa.

摘要

对35名感染人类免疫缺陷病毒1型（HIV-1）C亚型的埃塞俄比亚人进行了一项队列研究，以确定所有35名受试者的HLA表型以及参与跨亚型细胞介导免疫的高度保守的Gag蛋白区域。测定了15名个体的全长Gag病毒序列。通过干扰素-γ酶联免疫斑点试验检测CD8细胞介导的免疫反应。HLA-A03、-B49和-B57等位基因频率相对高于其他非洲人群。在最多数量的个体中可检测到抗p17（氨基酸1-60）CD8+。在50%的受试对象中检测到针对B亚型和C亚型的抗p17（氨基酸1-60和51-110）跨亚型反应。发现p24 KF11（氨基酸162-172）表位在HLA-B5703阳性个体中具有免疫优势。这些数据是关于感染埃塞俄比亚人HLA表型与HIV特异性细胞介导免疫反应相关性的首次报告，可能有助于为非洲这一地区设计诱导细胞毒性T淋巴细胞的疫苗。