Liu Hong-Wei, Hong Kun-Xue, Yuan Yuan, Liu Chun-Hua, Chen Jian-Ping, Ruan Yu-Hua, Wang Zhe, Shao Yi-Ming
Center for AIDS/STD Control and Prevention, Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Oct;25(5):358-60.
To characterize the Gag-Specific T lymphocyte responses and identify immunodominant region recognized in Chinese HIV-1 recombinant subtype B/C infectors.
10 antiretroviral treatment (ART) naive HIV-1 recombinant subtype B/C infectors with infected time in 1 year, 25 ART-naive infectors with infected time > 3 years and 10 HIV-1-seronegative healthy individuals were enrolled. HIV-1-specific T lymphocyte responses were analyzed by an IFN-gamma Elispot assay against 123 overlapping peptides spanning HIV-1 Gag protein in the present study.
Gag-specific T lymphocyte responses of interferon-gamma secretion were identified in 8(8/10) Chinese HIV-1 recombinant subtype B/ C infectors with infected time in 1 year, the specific T lymphocytes are mainly targeted at five seperated peptides. Responses were identified in 17(68%) infectors with infected time more than 3 years, the specific T lymphocytes are mainly targeted at one peptide in p17 and six in p24. There was obviously positive correlation (P = 0.0318, r = 0.519) between the magnitude of responses and viremia in infectors infected time > 3 years. The magnitude of response in infectors infected in 1 year was significantly higher than group infected time > 3 years (P = 0.021). None of healthy individuals produced positive responses.
HIV-1 recombinant subtype B/C Infectors at different stages of diseases recognize different region of gag.
描述中国HIV-1重组B/C亚型感染者中针对Gag的特异性T淋巴细胞反应，并确定其识别的免疫优势区域。
招募10名感染时间在1年以内的未接受抗逆转录病毒治疗（ART）的HIV-1重组B/C亚型感染者、25名感染时间超过3年的未接受ART的感染者以及10名HIV-1血清学阴性的健康个体。在本研究中，通过针对覆盖HIV-1 Gag蛋白的123个重叠肽段的γ干扰素酶联免疫斑点试验分析HIV-1特异性T淋巴细胞反应。
在8名（8/10）感染时间在1年以内的中国HIV-1重组B/C亚型感染者中检测到γ干扰素分泌的Gag特异性T淋巴细胞反应，特异性T淋巴细胞主要靶向5个分离的肽段。在17名（68%）感染时间超过3年的感染者中检测到反应，特异性T淋巴细胞主要靶向p17中的1个肽段和p24中的6个肽段。在感染时间超过3年的感染者中，反应强度与病毒血症之间存在明显的正相关（P = 0.0318，r = 0.519）。感染时间在1年以内的感染者的反应强度显著高于感染时间超过3年的组（P = 0.021）。健康个体均未产生阳性反应。
处于不同疾病阶段的HIV-1重组B/C亚型感染者识别gag的不同区域。
