Lichtenfeld Heinz, Stechemesser Hansjoachim, Möhwald Helmuth
Research Group for Colloids and Interfaces, Institute for Ceramics, Glass and Construction Materials, Technische Universität Bergakademie Freiberg, Freiberg, Germany.
J Colloid Interface Sci. 2004 Aug 1;276(1):97-105. doi: 10.1016/j.jcis.2004.03.038.
Present developments in the field of bioscience will profit from the fabrication of multilayer films of polyelectrolytes on colloids as well as of nano- and microsized capsules. The single particle light-scattering technique is suitable for process control. This paper describes the mode of operation and efficiency of a laboratory-built single particle light-scattering photometer, which opens up new possibilities in applications, especially in the field of bioscience. This has been achieved by improving the measurement capabilities of organic particle sizes near 100 nm and precision of thickness changes of about 0.5 nm. Besides the classic applications, such as particle size distribution measurements at high resolution and the coagulation kinetics of colloids, the measurement of the layer-by-layer polyelectrolyte adsorption at colloid particle surfaces and the fabrication of colloidal carriers is introduced. The described method is also a precise method for controlling the removal of the latex core to obtain multilayer microcapsules.
生物科学领域当前的发展将受益于在胶体以及纳米和微米级胶囊上制备聚电解质多层膜。单粒子光散射技术适用于过程控制。本文描述了实验室构建的单粒子光散射光度计的操作模式和效率，这为应用开辟了新的可能性，特别是在生物科学领域。这是通过提高对接近100 nm的有机颗粒尺寸的测量能力以及约0.5 nm的厚度变化精度来实现的。除了经典应用，如高分辨率粒度分布测量和胶体的凝聚动力学外，还介绍了胶体颗粒表面逐层聚电解质吸附的测量以及胶体载体的制备。所描述的方法也是一种精确控制去除乳胶核以获得多层微胶囊的方法。
