Poels Jeroen, Franssens Vanessa, Van Loy Tom, Martinez Alberto, Suner Marie-Marthe, Dunbar Stuart J, De Loof Arnold, Vanden Broeck Jozef
Laboratory for Developmental Physiology, Genomics and Proteomics, Naamsestraat 59, B-3000 Leuven, Belgium.
Biochem Biophys Res Commun. 2004 Jul 23;320(2):318-24. doi: 10.1016/j.bbrc.2004.05.165.
Activation or inhibition of the cyclic AMP (cAMP)-protein kinase A (PKA) pathway can ultimately regulate the transcription of a variety of genes. In vertebrates, the best characterized nuclear targets of PKA are the 'cAMP response element' (CRE) binding proteins (CREB). Differences in the transcriptional response to this pathway between cells and tissues can be based on the presence of distinct CREB isoforms. In this context, we have now investigated the presence of different dCREB transcripts in a stable, embryonic insect cell line, i.e., Drosophila Schneider 2 (S2) cells. In addition, we have studied the possible effect of cellular cAMP- and Ca2+ increases on the expression of a luciferase reporter in cells transfected with a CRE-containing reporter gene construct. In combination with recent data from the literature, our results indicate that the regulation of CRE-dependent gene expression shows some important differences between insects and vertebrates.
环磷酸腺苷（cAMP）-蛋白激酶A（PKA）信号通路的激活或抑制最终可调控多种基因的转录。在脊椎动物中，PKA最典型的核靶点是“cAMP反应元件”（CRE）结合蛋白（CREB）。细胞和组织对该信号通路转录反应的差异可能基于不同CREB亚型的存在。在此背景下，我们现已研究了稳定的胚胎昆虫细胞系，即果蝇施奈德2（S2）细胞中不同dCREB转录本的存在情况。此外，我们还研究了细胞内cAMP和Ca2+增加对转染含CRE报告基因构建体的细胞中荧光素酶报告基因表达的可能影响。结合近期文献数据，我们的结果表明，昆虫和脊椎动物在CRE依赖的基因表达调控方面存在一些重要差异。
