Maranesi Magda, Bochicchio Davide, Zambonin Laura, Tolomelli Brunella, Cabrini Luciana
Dipartimento di Biochimica G.Moruzzi, Università degli Studi di Bologna, via Irnerio 48 40126 Bologna, Italy.
J Nutr Biochem. 2004 Jul;15(7):396-401. doi: 10.1016/j.jnutbio.2003.09.006.
Our previous report demonstrated that, when vitamin deficiency is associated with high contents of long chain polyunsaturated fatty acids (LCPUFA) n3, lipid peroxidation susceptibility in rat heart and liver increases. In this paper, we evaluated the effect of the same dietary administration on lipid composition and antioxidant defenses of rat kidney. Results showed that vitamin B(6) deficiency, when associated with a fish oil diet, as compared to vegetable oil condition, increased relative kidney weight and decreased pyridoxal-5P contents. The different LCPUFA n3 dietary contents produced, on kidney phospholipids, effects interlaced with those of vitamin B(6) deficiency; in particular fish oil and vitamin B(6) deficient diet caused a significant decrease of arachidonic acid showing that the processes of elongation and desaturation of linoleic acid were slowed. Also, peroxidation susceptibility was higher, as demonstrated both by increased TBARS formation and glutathione peroxidase activity, and by decreased vitamin E contents and reduced glutathione/oxidized glutathione ratio.
我们之前的报告表明，当维生素缺乏与长链多不饱和脂肪酸（LCPUFA）n3的高含量相关时，大鼠心脏和肝脏中的脂质过氧化敏感性会增加。在本文中，我们评估了相同饮食管理对大鼠肾脏脂质组成和抗氧化防御的影响。结果表明，与植物油情况相比，维生素B6缺乏与鱼油饮食相关时，会增加肾脏相对重量并降低磷酸吡哆醛-5P含量。不同的LCPUFA n3饮食含量对肾脏磷脂产生的影响与维生素B6缺乏的影响相互交织；特别是鱼油和维生素B6缺乏饮食导致花生四烯酸显著减少，表明亚油酸的延长和去饱和过程减缓。此外，过氧化敏感性更高，这通过丙二醛（TBARS）形成增加和谷胱甘肽过氧化物酶活性增加以及维生素E含量降低和还原型谷胱甘肽/氧化型谷胱甘肽比率降低得以证明。
