Saito M, Nakatsugawa K
Division of Food Science, National Institute of Health and Nutrition, Tokyo, Japan.
Int J Vitam Nutr Res. 1994;64(2):144-51.
Feeding a high fish oil diet (25 wt%) to rats for 14 days increased the content of docosahexaenoic acid (DHA) in liver lipids, and diminished the liver antioxidant defenses as measured by the contents of alpha-tocopherol, ascorbic acid and non-protein SH, the last of which is consisting mostly of glutathione, and the activity of selenium-dependent glutathione peroxidase (GSHPx). These changes in antioxidant defenses are suggested to be associated with an increased susceptibility of the liver to lipid peroxidation as assessed by thiobarbituric acid (TBA) reaction and chemiluminescence intensity. Serum TBA value was also high in the rats given high fish oil diet. The present results demonstrate that a high fish oil diet potentiates susceptibility of rats to lipid peroxidation and augments requirement for antioxidants to provide adequate protection.
给大鼠喂食高鱼油饮食（25 wt%）14天，会增加肝脏脂质中二十二碳六烯酸（DHA）的含量，并降低肝脏的抗氧化防御能力，这通过α-生育酚、抗坏血酸和非蛋白SH的含量来衡量，其中非蛋白SH主要由谷胱甘肽组成，以及硒依赖性谷胱甘肽过氧化物酶（GSHPx）的活性。通过硫代巴比妥酸（TBA）反应和化学发光强度评估，这些抗氧化防御的变化表明与肝脏对脂质过氧化的易感性增加有关。给予高鱼油饮食的大鼠血清TBA值也很高。目前的结果表明，高鱼油饮食会增强大鼠对脂质过氧化的易感性，并增加对抗氧化剂的需求以提供充分的保护。
