一种通过[14C]氯吉兰放射自显影术测定区域单胺氧化酶A的简便方法。

A convenient method for regional monoamine oxidase-A determination by [14C]clorgyline autoradiography.

作者信息

Murakami M, Kondoh Y, Weimin Y, Mizusawa S, Nakamichi H, Takahashi K, Sasaki H, Iida H, Miura S, Kanno I

机构信息

Department of Radiology, Research Institute for Brain and Blood Vessels-Akita, Japan.

出版信息

Int J Rad Appl Instrum B. 1992 Aug;19(6):619-26. doi: 10.1016/0883-2897(92)90096-h.

DOI:10.1016/0883-2897(92)90096-h

PMID:1522016

Abstract

The availability of clorgyline for regional monoamine oxidase-A (MAO-A) determination was examined using [14C]clorgyline in rat. [14C]Clorgyline was synthesized by the methylation reaction of N-desmethyl-clorgyline and [14C]methyliodide in dimethylformamide with high radiochemical yield. The MAO-A distribution map by autoradiography correlated with that by histochemical technique and its quantity was consistent with the calculated MAO-A amount based on previous reports. The combination of labeled clorgyline and autoradiographic technique will promise the quantitative measurement of regional MAO-A distribution.

摘要

在大鼠体内使用[14C]氯吉兰研究了用于区域单胺氧化酶A（MAO-A）测定的氯吉兰的可用性。[14C]氯吉兰是通过N-去甲基氯吉兰与[14C]甲基碘在二甲基甲酰胺中的甲基化反应合成的，具有较高的放射化学产率。通过放射自显影得到的MAO-A分布图与组织化学技术得到的分布图相关，其数量与根据先前报告计算出的MAO-A量一致。标记氯吉兰与放射自显影技术的结合将有望实现区域MAO-A分布的定量测量。