Marks J, Fankhauser C, Reymond A, Simanis V
Unité de Recherche sur le cycle cellulaire, ISREC, Epalinges, Switzerland.
J Cell Sci. 1992 Mar;101 ( Pt 3):517-28. doi: 10.1242/jcs.101.3.517.
The cdc10 gene of the fission yeast S. pombe is required for traverse of the start control in late G1 and commitment to the mitotic cell cycle. To increase our understanding of the events which occur at start, a pseudoreversion analysis was undertaken to identify genes whose products may interact with cdc10 or bypass the requirement for it. A single gene, sct1+ (suppressor of cdc ten), has been identified, mutation of which suppresses all conditional alleles and a null allele of cdc10. Bypass of the requirement for cdc10+ function by sct1-1 mutations leads to pleiotropic defects, including microtubule, microfilament and nuclear structural abnormalities. Our data suggest that sct1 encodes a protein that is dependent upon cdc10+ either for its normal function or expression, or is a component of a checkpoint that monitors execution of p85cdc10 function.
粟酒裂殖酵母的cdc10基因是G1晚期通过起始控制和进入有丝分裂细胞周期所必需的。为了增进我们对起始阶段发生事件的理解，进行了假回复分析以鉴定其产物可能与cdc10相互作用或绕过对它的需求的基因。已鉴定出一个单一基因sct1+（cdc10的抑制子），其突变可抑制所有条件等位基因和cdc10的无效等位基因。sct1-1突变绕过对cdc10+功能的需求会导致多效性缺陷，包括微管、微丝和核结构异常。我们的数据表明，sct1编码一种蛋白质，该蛋白质的正常功能或表达依赖于cdc10+，或者是监测p85cdc10功能执行情况的检查点的一个组成部分。
