Lowndes N F, McInerny C J, Johnson A L, Fantes P A, Johnston L H
Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK.
Nature. 1992 Jan 30;355(6359):449-53. doi: 10.1038/355449a0.
In the budding yeast Saccharomyces cerevisiae, cell-cycle control over DNA synthesis occurs partly through the coordinate expression in late G1 phase of many, if not all, of the genes required for DNA synthesis. A cis-acting hexamer element ACGCGT (an MluI restriction site) is responsible for coordinating transcriptional regulation of these genes at the G1/S phase boundary and we have identified a binding activity, DSC1, that recognizes these sequences in a cell-cycle-dependent manner. In the distantly related fission yeast Schizosaccharomyces pombe, only one of the known DNA synthesis genes, cdc22+, which encodes a subunit of ribonucleotide reductase, is periodically expressed in late G1 (ref. 6). The promoter region of cdc22+ has two MluI sites and five related sequences, suggesting that similar controls over DNA synthesis genes could occur in fission yeast. We report here a binding activity in fission yeast that is very similar to DSC1 in budding yeast. We also show that the fission yeast cdc10+ gene product, which is required for Start and entry into S phase, is a component of this binding activity.
在出芽酵母酿酒酵母中，对DNA合成的细胞周期控制部分是通过在G1期晚期协调表达许多（如果不是全部）DNA合成所需的基因来实现的。一个顺式作用的六聚体元件ACGCGT（一个MluI限制位点）负责在G1/S期边界协调这些基因的转录调控，并且我们已经鉴定出一种结合活性，即DSC1，它以细胞周期依赖性方式识别这些序列。在远缘相关的裂殖酵母粟酒裂殖酵母中，已知的DNA合成基因中只有一个，即cdc22+，它编码核糖核苷酸还原酶的一个亚基，在G1晚期周期性表达（参考文献6）。cdc22+的启动子区域有两个MluI位点和五个相关序列，这表明在裂殖酵母中可能对DNA合成基因进行类似的控制。我们在此报告裂殖酵母中一种与出芽酵母中的DSC1非常相似的结合活性。我们还表明，裂殖酵母中Start和进入S期所需的cdc10+基因产物是这种结合活性的一个组成部分。
