Huang Y, Wan H Q, Liu H Q, Wu Y T, Liu X F
Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, Yangzhou, P.R. China.
Arch Virol. 2004 Jul;149(7):1445-57. doi: 10.1007/s00705-004-0297-8. Epub 2004 Mar 25.
We have completely sequenced the genome of an isolate of Newcastle disease virus (NDV) obtained following an outbreak in geese. The genomic sequence consists of 15192 nt, which is six nt longer than the published full length genome of the NDV strains La Sota, B1, Clone-30, Beaudette C and HB V4. The six nt insertion was located in the non-coding region of the nucleoprotein (NP) gene between nt 1646 and nt 1647 of the NDV genome (numbered according to the genomic sequence of the La Sota strain). An additional 22 NDV strains were searched for the existence of this six nt insertion. NDVs in genotypes VI, VII, VIII and IX had this insertion while NDV's in genotypes I, II, III, IV, and V did not. The significance of this insertion in NDV evolution is discussed.
我们已对一株在鹅群爆发疫情后获得的新城疫病毒（NDV）分离株的基因组进行了全序列测定。该基因组序列由15192个核苷酸组成，比已发表的NDV毒株La Sota、B1、Clone - 30、Beaudette C和HB V4的全长基因组序列长6个核苷酸。这6个核苷酸的插入位于NDV基因组中核蛋白（NP）基因的非编码区，在NDV基因组的第1646和1647核苷酸之间（根据La Sota毒株的基因组序列编号）。另外对22株NDV进行了搜索，以查找这6个核苷酸插入的存在情况。基因型VI、VII、VIII和IX的NDV有此插入，而基因型I、II、III、IV和V的NDV则没有。本文讨论了该插入在NDV进化中的意义。
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