Fahy M M, Kane M T
Department of Physiology, University College, Galway, Ireland.
Hum Reprod. 1992 Apr;7(4):550-2. doi: 10.1093/oxfordjournals.humrep.a137688.
The effect of different concentrations (0, 0.6, 3, 15, 75 and 375 microM) of myo-inositol on the development of rabbit morulae to expanded blastocysts was investigated in terms of blastocyst expansion and synthesis of DNA and protein, as measured by incorporation of [3H]thymidine and [14C]amino acids into acid-precipitable material. A concentration of 15 microM inositol caused a 2.8-fold increase in blastocyst expansion (P less than 0.01), a 9.9-fold increase in thymidine incorporation into DNA (P less than 0.01) and a 3.6-fold increase in amino acid incorporation into protein (P less than 0.01). There were no significant differences in the range from 15 to 375 microM inositol.
通过将[3H]胸腺嘧啶核苷和[14C]氨基酸掺入酸沉淀物质中进行测量，从囊胚扩张以及DNA和蛋白质合成方面研究了不同浓度（0、0.6、3、15、75和375微摩尔）的肌醇对兔桑葚胚发育至扩张囊胚的影响。15微摩尔浓度的肌醇使囊胚扩张增加2.8倍（P<0.01），胸腺嘧啶核苷掺入DNA增加9.9倍（P<0.01），氨基酸掺入蛋白质增加3.6倍（P<0.01）。在15至375微摩尔肌醇范围内没有显著差异。
