Kane M T
Department of Physiology, University College, Galway, Ireland.
J Exp Zool. 1988 Feb;245(2):220-3. doi: 10.1002/jez.1402450211.
The vitamin requirements for culture of rabbit morulae to expanded blastocysts were examined. Early morulae were cultured for 5 days either in a control complete medium containing all the 11 water-soluble vitamins of F10 culture medium (biotin, pantothenate, choline, inositol, niacinamide, pyridoxine, riboflavin, thiamine, folic acid, B12, and lipoic acid) or in media with each vitamin omitted individually. Blastocyst diameters were measured at the end of culture. The omission of inositol, pyridoxine, riboflavin, and niacinamide resulted in large statistically significant decreases in blastocyst expansion. The omission of B12 resulted in a significant increase in blastocyst expansion indicating that the level present in F10 is toxic to rabbit blastocysts.
研究了兔桑椹胚培养至扩张囊胚所需的维生素。将早期桑椹胚在含有F10培养基中全部11种水溶性维生素（生物素、泛酸盐、胆碱、肌醇、烟酰胺、吡哆醇、核黄素、硫胺素、叶酸、维生素B12和硫辛酸）的对照完全培养基中培养5天，或在单独省略每种维生素的培养基中培养5天。在培养结束时测量囊胚直径。省略肌醇、吡哆醇、核黄素和烟酰胺导致囊胚扩张在统计学上显著大幅下降。省略维生素B12导致囊胚扩张显著增加，这表明F10中存在的该水平对兔囊胚有毒性。
