含鳖甲煎口服液大鼠血清对大鼠肝星状细胞增殖的影响
Effect of rat serum containing Biejiajian oral liquid on proliferation of rat hepatic stellate cells.
作者信息
Yao Li, Yao Zhen-Min, Weng Heng, Zhao Ge-Ping, Zhou Yue-Jun, Yu Tao
机构信息
Department of Pharmacology, Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, Zhejiang Province, China.
出版信息
World J Gastroenterol. 2004 Jul 1;10(13):1911-3. doi: 10.3748/wjg.v10.i13.1911.
AIM
Liver fibrosis is a common pathological process of chronic liver diseases. Activation of hepatic stellate cells (HSCs) is the key issue in the occurrence of liver fibrosis. In this study, we observed the inhibitory action of rat serum containing Biejiajian oral liquid (BOL), a decoction of turtle shell, on proliferation of rat HSCs, and to explore the anti-hepatofibrotic mechanisms of BOL.
METHODS
A rat model of hepatic fibrosis was induced by subcutaneous injection of CCl(4). Serum containing low, medium and high dosages of BOL was prepared respectively. Normal and fibrotic HSCs were isolated and cultured. The effect of sera containing BOL on proliferation of HSCs was determined by (3)H-TdR incorporation.
RESULTS
The inhibitory rate of normal rat HSC proliferation caused by 100 mL/mL sera containing medium and high dosages of BOL showed a remarkable difference as compared with that caused by colchicine (medium dosage group: 34.56+/-4.21% vs 29.12+/-2.85%, P<0.01; high dosage group: 37.82+/-1.32% vs 29.12+/-2.85%, P<0.01). The inhibitory rate of fibrotic rat HSC proliferation caused by 100 mL/L serum containing medium and high dosages of BOL showed a remarkable difference as compared with that caused by colchicine (medium dosage group: 51.31+/-3.14% vs 38.32+/-2.65%, P<0.01; high dosage group: 60.15+/-5.36% vs 38.32+/-2.65%, P<0.01). The inhibitory rate of normal rat HSC proliferation caused by 100 mL/L and 200 mL/L sera containing a medium dosage of BOL showed a significant difference as compared with that caused by 50 mL/L (100 mL/L group: 69.02+/-9.96% vs 50.82+/-9.28%, P<0.05; 200 mL/L group: 81.78+/-8.92% vs 50.82+/-9.28%, P<0.01). The inhibitory rate of fibrotic rat HSC proliferation caused by 100 mL/L and 200 mL/L sera containing a medium dosage of BOL showed a significant difference as compared with that caused by 50 mL/L (100 mL/L group: 72.19+/-10.96% vs 61.38+/-7.16%, P<0.05; 200 mL/L group: 87.16+/-8.54% vs 61.38+/-7.16%, P<0.01).
CONCLUSION
Rat serum containing BOL can inhibit proliferation of rat HSCs, and the inhibition depends on the dosage and concentration of BOL. The inhibitory effect on HSC proliferation is one of the main anti-hepatofibrotic mechanisms of BOL.
目的
肝纤维化是慢性肝病常见的病理过程。肝星状细胞（HSCs）的激活是肝纤维化发生的关键问题。本研究观察含鳖甲煎口服液（BOL，一种龟甲汤剂）的大鼠血清对大鼠肝星状细胞增殖的抑制作用，并探讨BOL的抗肝纤维化机制。
方法
通过皮下注射四氯化碳诱导大鼠肝纤维化模型。分别制备含低、中、高剂量BOL的血清。分离并培养正常和纤维化的肝星状细胞。通过³H-TdR掺入法测定含BOL血清对肝星状细胞增殖的影响。
结果
含中、高剂量BOL的100 mL/mL血清对正常大鼠肝星状细胞增殖的抑制率与秋水仙碱相比有显著差异（中剂量组：34.56±4.21%对29.12±2.85%，P<0.01；高剂量组：37.82±1.32%对29.12±2.85%，P<0.01）。含中、高剂量BOL的100 mL/L血清对纤维化大鼠肝星状细胞增殖的抑制率与秋水仙碱相比有显著差异（中剂量组：51.31±3.14%对38.32±2.65%，P<0.01；高剂量组：60.15±5.36%对38.32±2.65%，P<0.01）。含中剂量BOL的100 mL/L和200 mL/L血清对正常大鼠肝星状细胞增殖的抑制率与50 mL/L相比有显著差异（100 mL/L组：69.02±9.96%对50.82±9.28%，P<0.05；200 mL/L组：81.78±8.92%对50.82±9.28%，P<0.01）。含中剂量BOL的100 mL/L和200 mL/L血清对纤维化大鼠肝星状细胞增殖的抑制率与50 mL/L相比有显著差异（100 mL/L组：72.19±10.96%对61.38±7.16%，P<0.05；200 mL/L组：87.16±8.54%对61.38±7.16%，P<0.01）。
结论
含BOL的大鼠血清可抑制大鼠肝星状细胞的增殖，且抑制作用取决于BOL的剂量和浓度。对肝星状细胞增殖的抑制作用是BOL抗肝纤维化的主要机制之一。
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