Teles M, Santos Maria A, Pacheco M
Animal Physiology/Ecotoxicology Sector, Biology Department, Aveiro University, 3810-193 Aveiro, Portugal.
Ecotoxicol Environ Saf. 2004 Jul;58(3):373-8. doi: 10.1016/j.ecoenv.2004.01.006.
European eels (Anguilla anguilla L.) were caged for 8 and 48 h in two polluted areas to assess the contamination cleanup process in the Vouga River 2 years after the official closing of a bleached kraft pulp mill effluent (BKPME; experiment 1), and to monitor the effects induced by contaminated offward fishing harbor waters (experiment 2). Plasma cortisol, glucose, and lactate were evaluated as stress responses. In experiment 1, plasma cortisol, glucose, and lactate increased after 8 h of exposure in site 3, which is located farthest from the deactivated sewage outlet. However, A. anguilla seemed to adapt after 48 h of exposure in site 3, because all three parameters returned to control levels. Plasma glucose also significantly increased after 8 h of exposure at sites 1, 2, and 3, returning to control levels after 48 h. Plasma lactate levels increased after 8 h of exposure at site 3 and after 48 h of exposure at site 1. In experiment 2, A. anguilla exposed to contaminated harbor waters increased their plasma lactate after 8 and 48 h of exposure, whereas their cortisol and glucose plasma were elevated only after 48 h of exposure. The results demonstrate that even 2 years after the official closing of the BKPME sewage outlet, the river Vouga water remains contaminated by the sediment associated chemicals. Because the fishing harbor induced in A. anguilla the same type of stress responses, it is also an area of concern. The adopted stress parameters allied to a caging strategy are recommended for future environmental monitoring assessments.
将欧洲鳗鲡（Anguilla anguilla L.）置于两个污染区域中8小时和48小时，以评估一家漂白硫酸盐浆厂废水排放口正式关闭两年后沃加河的污染清理进程（实验1），并监测受污染的外港水域所引发的影响（实验2）。评估血浆皮质醇、葡萄糖和乳酸水平作为应激反应指标。在实验1中，位于离停用污水排放口最远的3号地点，暴露8小时后血浆皮质醇、葡萄糖和乳酸水平升高。然而，欧洲鳗鲡在3号地点暴露48小时后似乎出现了适应现象，因为所有这三个参数都恢复到了对照水平。在1号、2号和3号地点暴露8小时后血浆葡萄糖水平也显著升高，48小时后恢复到对照水平。在3号地点暴露8小时以及在1号地点暴露48小时后血浆乳酸水平升高。在实验2中，暴露于受污染港口水域的欧洲鳗鲡在暴露8小时和48小时后血浆乳酸水平升高，而其血浆皮质醇和葡萄糖仅在暴露48小时后升高。结果表明，即使在漂白硫酸盐浆厂废水排放口正式关闭两年后，沃加河的水仍受到与沉积物相关化学物质的污染。由于渔港在欧洲鳗鲡身上引发了相同类型的应激反应，它也是一个值得关注的区域。建议将所采用的应激参数与网箱策略相结合，用于未来的环境监测评估。
