Zák J, Kapitola J, Povýsil C
Geriatrická klinika, 1. lékarské fakulty Univerzity Kariovy a Vseobecné fakultní nemocnice, Na Bojisti 1, 120 00 Praha 2, Czech Republic.
Sb Lek. 2003;104(3):293-7.
Authors deal with question, if there is possibility to infer bone histological structure (described by histomorphometric parameters of trabecular bone volume and trabecular thickness) from bone density, ash weight or even from weight of animal (rat). Both tibias of each of 30 intact male rats, 90 days old, were processed. Left tibia was utilized to the determination of histomorphometric parameters of undecalcified bone tissue patterns by automatic image analysis. Right tibia was used to the determination of values of bone density, using Archimedes' principle. Values of bone density, ash weight, ash weight related to bone volume and animal weight were correlated with histomorphometric parameters (trabecular bone volume, trabecular thickness) by Pearson's correlation test. One could presume the existence of relation between data, describing bone mass at the histological level (trabecular bone of tibia) and other data, describing mass of whole bone or even animal mass (weight). But no statistically significant correlation was found. The reason of the present results could be in the deviations of trabecular density in marrow of tibia. Because of higher trabecular bone density in metaphyseal and epiphyseal regions, the histomorphometric analysis of trabecular bone is preferentially done in these areas. It is possible, that this irregularity of trabecular tibial density could be the source of the deviations, which could influence the results of correlations determined. The values of bone density, ash weight and animal weight do not influence trabecular bone volume and vice versa: static histomorphometric parameters of trabecular bone do not reflect bone density, ash weight and weight of animal.
作者探讨了是否有可能从骨密度、灰重甚至动物（大鼠）体重推断骨组织学结构（由小梁骨体积和小梁厚度的组织形态计量学参数描述）。对30只90日龄完整雄性大鼠的双侧胫骨进行处理。左侧胫骨用于通过自动图像分析测定未脱钙骨组织模式的组织形态计量学参数。右侧胫骨用于利用阿基米德原理测定骨密度值。通过Pearson相关检验将骨密度、灰重、与骨体积相关的灰重以及动物体重的值与组织形态计量学参数（小梁骨体积、小梁厚度）进行关联。人们可以推测，在组织学水平描述骨量的数据（胫骨小梁骨）与描述整个骨量甚至动物体重的数据之间存在关联。但未发现统计学上的显著相关性。当前结果的原因可能在于胫骨骨髓中小梁密度的偏差。由于干骺端和骨骺端区域的小梁骨密度较高，因此小梁骨的组织形态计量学分析优先在这些区域进行。有可能胫骨小梁密度的这种不规则性可能是偏差的来源，这可能会影响所确定的相关性结果。骨密度、灰重和动物体重的值不会影响小梁骨体积，反之亦然：小梁骨的静态组织形态计量学参数不能反映骨密度、灰重和动物体重。
