Szabo S E, Monroe S L, Fiorino S, Bitzan J, Loper K
The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Hospital, Baltimore, Maryland, USA.
Lab Hematol. 2004;10(2):109-11. doi: 10.1532/LH96.04020.
Two important parameters for determination of deleterious effects of cellular processing on hematopoietic progenitor cells are cell viability and concentration. The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins Hospital evaluated the Beckman Coulter Vi-Cell automated instrument for the measurement of these two parameters. Using 33 thawed hematopoietic progenitor cell samples, automated Vi-Cell viability results were compared to those obtained using the standard trypan blue manual method. In addition, cell concentrations from these samples were compared with results from the Model Z2 Coulter Counter. Chinese Hamster Ovary cells were used for the evaluation of Vi-Cell linearity at the Beckman Coulter Cellular Analysis Development Center. Significant correlation was obtained when the two methods were compared for both cell concentration and percentage viability (P < .0001). The results of the linearity study indicated that the Vi-Cell is linear from approximately 5 x 10(4) to greater than 1 x 10(7) cells/mL. The Vi-Cell uses sample volumes as low as 0.5 mL; cell diameters may be 2 to 70 microns. The Vi-Cell automated instrument offers many significant advantages for cell analyses in today's busy laboratory environment.
用于确定细胞处理对造血祖细胞有害影响的两个重要参数是细胞活力和浓度。约翰霍普金斯医院的西德尼·金梅尔综合癌症中心评估了贝克曼库尔特Vi-Cell自动仪器对这两个参数的测量。使用33个解冻的造血祖细胞样本，将Vi-Cell自动测量的活力结果与使用标准台盼蓝手动方法获得的结果进行比较。此外，将这些样本的细胞浓度与Z2型库尔特计数器的结果进行比较。在中国仓鼠卵巢细胞用于在贝克曼库尔特细胞分析开发中心评估Vi-Cell线性。当比较两种方法的细胞浓度和活力百分比时，获得了显著相关性（P <.0001）。线性研究结果表明，Vi-Cell在大约5 x 10(4)至大于1 x 10(7)个细胞/mL范围内呈线性。Vi-Cell使用低至0.5 mL的样本体积；细胞直径可能为2至70微米。在当今繁忙的实验室环境中，Vi-Cell自动仪器为细胞分析提供了许多显著优势。
