Michaud P, Da Costa A, Courtois B, Courtois J
Laboratoire des Glucides--LPMV, IUT/Génie Biologique, Université de Picardie Jules Verne, Avenue des Facultés, Le Bailly, 80025 Amiens Cedex, France.
Crit Rev Biotechnol. 2003;23(4):233-66. doi: 10.1080/07388550390447043.
Polysaccharide lyases, which are polysaccharide cleavage enzymes, act mainly on anionic polysaccharides. Produced by prokaryote and eukaryote organisms, these enzymes degrade (1,4) glycosidic bond by a beta elimination mechanism and have unsaturated oligosaccharides as major products. New polysaccharides are cleaved only by their specific polysaccharide lyases. From anionic polysaccharides controlled degradations, various biotechnological applications were investigated. This review catalogues the degradation of bacterial, plant and animal polysaccharides (neutral and anionic) by this family of carbohydrate acting enzymes.
多糖裂解酶是一类多糖切割酶，主要作用于阴离子多糖。这些酶由原核生物和真核生物产生，通过β-消除机制降解（1,4）糖苷键，主要产物为不饱和寡糖。新的多糖只能被其特定的多糖裂解酶切割。通过对阴离子多糖的可控降解，人们研究了各种生物技术应用。这篇综述编目了这类碳水化合物作用酶对细菌、植物和动物多糖（中性和阴离子）的降解情况。
