Linhardt R J
University of Iowa, Iowa City, Iowa, USA.
Curr Protoc Mol Biol. 2001 May;Chapter 17:Unit17.13B. doi: 10.1002/0471142727.mb1713bs48.
Polysaccharide lyases are a class of enzymes useful for analysis of glycosaminoglycans (GAGs) and the glycosaminoglycan component of proteoglycans (PGs). These enzymes cleave specific glycosidic linkages present in acidic polysaccharides and result in depolymerization. The lyases are derived from a wide variety of pathogenic and nonpathogenic bacteria and fungi. This class of enzymes includes heparin lyases (heparinases), heparan sulfate lyases (heparanases or heparitinases), chondroitin lyases (chondroitinases), and hyaluronate lyases (hyaluronidases), all of which are described in this unit. Two protocols describe depolymerization of GAGs, and two support protocols describe assays to confirm and quantitate the activity of heparin and chondroitin ABC lyases.
多糖裂解酶是一类对分析糖胺聚糖（GAGs）和蛋白聚糖（PGs）的糖胺聚糖成分有用的酶。这些酶能切割酸性多糖中存在的特定糖苷键并导致解聚。这些裂解酶来源于多种致病和非致病细菌及真菌。这类酶包括肝素裂解酶（肝素酶）、硫酸乙酰肝素裂解酶（乙酰肝素酶或类肝素酶）、软骨素裂解酶（软骨素酶）和透明质酸裂解酶（透明质酸酶），本单元将对所有这些酶进行描述。两个方案描述了GAGs的解聚，两个支持方案描述了用于确认和定量肝素及软骨素ABC裂解酶活性的测定方法。
