Heber-Katz Ellen, Chen Pan, Clark Lise, Zhang Xiang-Ming, Troutman Scott, Blankenhorn Elizabeth P
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA.
Wound Repair Regen. 2004 May-Jun;12(3):384-92. doi: 10.1111/j.1067-1927.2004.012308.x.
The MRL mouse has been shown to display an epimorphic regenerative response after ear hole punching leading to complete closure within 30 days and cartilage regrowth. The regenerative capacity of the MRL has also been seen after a severe cryoinjury to the heart leads to complete healing without scarring and functional myocardium. The wound healing ear hole closure response that occurs in MRL mice has been shown to be genetically controlled. We have previously identified 11 quantitative trait loci (QTL) that govern healing in an intercross of (MRL x C57BL/6 J) mice. However, it is desirable to use another poorly healing mouse strain to elucidate the full range of genetic factors that affect this important process. In the current study, we have used an inbred subspecies of the mouse, M. castaneus, and have confirmed a number of loci identified previously. In addition, we report three new healing QTL. Furthermore, in this strain combination, we note a strong sexual dimorphism also observed in the MRL x C57BL/6 cross, both in the healing trait and in the QTL that control it.
MRL小鼠在耳打孔后已显示出具有再生性的修复反应，能在30天内完全闭合且有软骨再生。在心脏遭受严重冷冻损伤后，MRL小鼠也表现出再生能力，可实现完全愈合且无疤痕形成，心肌功能恢复正常。已证明MRL小鼠出现的伤口愈合耳孔闭合反应受基因控制。我们之前在（MRL×C57BL/6 J）小鼠的杂交后代中鉴定出11个控制愈合的数量性状基因座（QTL）。然而，使用另一种愈合能力较差的小鼠品系来阐明影响这一重要过程的所有遗传因素是很有必要的。在当前研究中，我们使用了小鼠的一个近交亚种——栗色小鼠（M. castaneus），并证实了一些先前鉴定出的基因座。此外，我们报告了三个新的愈合QTL。此外，在这种品系组合中，我们注意到在MRL×C57BL/6杂交中也观察到的强烈的性别二态性，这在愈合性状以及控制该性状的QTL中均有体现。
